Question (23038) | Anti-EGFR antibody [E114] (ab32562)

Go to datasheet (ab32562)

Question

Dear technical support: This customer raised an enquire about ab32562, she would like to ask for your help to know the approximate concentration range of this antibody, because the online datasheet show the “concentration not determined”, could you please help this customer to solve the problem? Thanks for your kindly assistance. Best regards

Answer

Thank you for your enquiry. I can confirm that because ab32562 Anti-EGFR antibody [E114] is provided as cell culture supernatant, the concentration of antibody has not been determined. Antibody concentration is usually assessed by measuring protein concentration of the solution. In the case of tissue culture supernatant, this will contain many other proteins as well as antibody, and this means it is not possible to obtain an accurate result for this. We can recommend that tissue culture supernatant is known to contain approximately 1 - 3 mg/ml of antibody. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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