• Product name

    Anti-EGFR antibody [EGFR1] (Biotin)
    See all EGFR primary antibodies
  • Description

    Mouse monoclonal [EGFR1] to EGFR (Biotin)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human epidermoid carcinoma cells A431.

  • Epitope

    Recognizes the extracellular domain of the epidermal growth factor receptor on many cell surfaces.



Our Abpromise guarantee covers the use of ab24293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.


  • Function

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificity

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in disease

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • Cellular localization

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • Database links

  • Alternative names

    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all


  • ab24293, at a 1/45 dilution, staining EGFR in human glioblastoma U87MGwtEGFR cells by Flow Cytometry analysis. The image shows cells with (blue) and without (red) EGFR1-biotin primary antibody. In lieu of a secondary antibody, a streptavidin-R-phycoerythrin conjugate was used as a secondary reagent at a 1/83 dilution.

    See Abreview


This product has been referenced in:

  • Park J  et al. Analyses of Intravesicular Exosomal Proteins Using a Nano-Plasmonic System. ACS Photonics 5:487-494 (2018). Read more (PubMed: 29805987) »
  • Burel-Vandenbos F  et al. Cells with intense EGFR staining and a high nuclear to cytoplasmic ratio are specific for infiltrative glioma: a useful marker in neuropathological practice. Neuro Oncol 15:1278-88 (2013). Read more (PubMed: 23935154) »
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (H292 and MTEC(mouse trachea epithelial cells, wt a)
Yes - 15min 1%BSA/0.2%Triton X-100/PBS
H292 and MTEC(mouse trachea epithelial cells, wt a
Blocking step
NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Mrs. Aida Habibovic

Verified customer

Submitted Nov 29 2017


Thank you for your inquiry.

For ab24293, the laboratory has stated that the biotinylation procedure is proprietary. The only information they will release is that the procedure is designed to assure the degree of biotinylation is no greater than 8.

I apologize that this information could not be more helpful.

Read More


Thank you for your inquiry.

I heard back from the lab that unfortunately this has not been tested so the information is not available.
I'm sorry that we couldn't be of more help in this regard, but please let me know if you have any additional questions.

Read More


Thank you for your reply.

The antibody ab30 will be covered under our Abpromise guarantee for use in ICC on human cells. If for any reason you are unsatisfied with the results, we will be happy to refund or replace it for you as long as you contact us within six months of purchase. I hope this helps, pleaselet me know if you have any additional questionsor concerns.

Read More


Thank you for your enquiry and your interest in our products.

I can confirm that neither of these antibodies have been characterized for inhibition studies. ab24284 is suitable for IHC-P only; whilst ab24293 has been tested for Flow cytometry and IHC-Fr.

I have conducted a search for you and found another product in our catalogue ab231 which has been tested for inhibition studies. I would advise you to take a look at the on-line datasheet to see if it suits your need:


I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More


Thank you for your call today and for our discussion of these EGFR antibodies.

Please let me know if you have any further questions or would like any assistance choosing reagents for your studies.

I hope you have a great day!

Read More
Flow Cytometry
Human Cell (U87MG.wtEGFR)
Cell harvesting/tissue preparation method: cell line
Sample buffer: PBS + 1 g/L BSA
not fixed
Gating Strategy
forward scatter and side scatter

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Verified customer

Submitted Aug 27 2008

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