Question (65942) | Anti-EGFR antibody [EGFR1] (Phycoerythrin) (ab130738)

Go to datasheet (ab130738)

Question


Could you pls advise me on the best pair of florescently labeled
antibodies that you sell to use for FACS to distinguish 3T3 and A549
(lung, human) cells?

Answer

Thank you for your interesting question. We have some antibodies that recognize one species but not the other, but not all the targets for these antibodies are known to be expressed on these cell lines. What I found were two candidates:

ab15680 - Detects MHC class I of the 3T3 haplotype (H-2q), but not human MHC.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=15680).

However, this antibody is not conjugated to a fluorochrome so it will require a secondary antibody such as ab98386:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98386).

For detection of a protein on A549, I recommend anti-EGFR, ab130738. This is conjugated to phycoerythrin.

Using these two antibodies, you should be able to sort A549 from 3T3. However, we do not guarantee exclusive species reactivity. In the past, it has been observed that ab15680 does not react with human cells and ab130738 does not react with mouse cells, but neither has been tested against these two particular cell lines.

I hope this helps. Please let us know if you have any other questions.

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