Recombinant Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-338479-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750)
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EGFR aa 700-800 (deleted E746 + L747 + R748 + E749 + A750). The exact sequence is proprietary.
Database link: P00533 -
Positive control
- IHC-P: Human lung carcinoma tissue. WB: HCC827 cell lysate. ICC/IF: HCC827 cells Flow Cyt: HCC827 cells
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP111 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab227737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
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| ICC/IF | 1/1000. | |
| Flow Cyt | 1/400. | |
| WB | 1/300. Predicted molecular weight: 134 kDa. Primary antibody incubation for 1 hour at room temperature. |
Target
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Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
Involvement in disease
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
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Database links
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
- Unigene: 605083 Human
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Alternative names
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Immunocytochemistry/ Immunofluorescence analysis of HCC827(human lung adenocarcinoma epithelial cell), negative control:
A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:100 (4.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-338499-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-flow-cytometry.jpg)
Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Flow Cytometry analysis of HCC827(Human lung adenocarcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:400 dilution (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-301577-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for EGFR (deleted E746 + L747 + R748 + E749 + A750) using ab227737 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-301576-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-western-blot.jpg)
Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737) at 1/300 dilution + HCC827 cell lysate
Predicted band size: 134 kDa -
![Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-5-benefits-of-recombinant-antibodies.png)
Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab227737 has not yet been referenced specifically in any publications.
