• Product name

    Anti-EGFR (phospho S1142) antibody
    See all EGFR primary antibodies
  • Description

    Rabbit polyclonal to EGFR (phospho S1142)
  • Host species

  • Specificity

    ab76194 is not predicted to react with other ErbB family members.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human EGFR conjugated to Keyhole Limpet Haemocyanin (KLH). The aa numbering is around serine 1166 if the signal peptide is included.
    Database link: P00533

  • Positive control

    • IHC-P: A431 cells untreated or treated with Calyculin A (50 nM) for 15 minutes. WB: A431 cell lystes treated with Calyculin A (100 nM) for 30 minutes.



Our Abpromise guarantee covers the use of ab76194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/2000.
ICC/IF 1/200.


  • Function

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificity

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in disease

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • Cellular localization

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • Database links

  • Alternative names

    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all


  • ab76194, at a 1/200 dilution, staining EGFR in A431 cells by Immunoflurescence.
    Left image: untreated.
    Middle image: treated with Calyculin A (50 nM) for 15 minutes.
    Right image: treated with Calyculin A (50 nM) for 15 minutes with blocking peptide, phospho EGFR (Ser-1142).
  • All lanes : Anti-EGFR (phospho S1142) antibody (ab76194) at 1/1000 dilution

    Lane 1 : A431 cells treated with
    Calyculin A (100 nM) for 30 minutes
    Lane 2 : A431 cells treated with
    Calyculin A (100 nM) for 30 minutes and then treated with lambda phosphatase

    Predicted band size: 134 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?


ab76194 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your recent telephone enquiry which I have investigated further. As discussed on the telephone, there is a lot of post translational modification on this protein according to SwissProt/Uniprot site, which indicates that its weight on a western blot could be much higher than expected. http://www.uniprot.org/uniprot/P00533 I am sorry we are not aware of any current publications for this specific antibody.  In many cases, regrettably we do not have access to articles mentioning our products, or subscriptions to the journals that publish them. But there are other publications that have detected EFFR at 180 kDa on western blot: http://www.jbc.org/content/278/37/35781.full.pdf http://endo.endojournals.org/content/137/3/905.abstract?related-urls=yes&legid=endo;137/3/905   I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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