Recombinant Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
![Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.com/ps/products/32/ab32578/Images/ab32578-478748-anti-egfr-phospho-y1173-antibody-e124-westernblot-A431-human.jpg "Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E124] to EGFR (phospho Y1173)
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EGFR (phospho Y1173) antibody [E124]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [E124] to EGFR (phospho Y1173) -
Host species
Rabbit -
Specificity
The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor) -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (Intra): Trichostatin A treated HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E124 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
- Alexa Fluor® 594 Anti-EGFR (phospho Y1173) antibody [E124] (ab207020)
- Alexa Fluor® 405 Anti-EGFR (phospho Y1173) antibody [E124] (ab207235)
- Alexa Fluor® 555 Anti-EGFR (phospho Y1173) antibody [E124] (ab207236)
- Alexa Fluor® 488 Anti-EGFR (phospho Y1173) antibody [E124] (ab207345)
- Alexa Fluor® 647 Anti-EGFR (phospho Y1173) antibody [E124] (ab207346)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/25 - 1/50.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).
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| Notes |
|---|
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ICC/IF
1/25 - 1/50. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa). |
Target
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Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
Involvement in disease
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
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Database links
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
- Unigene: 605083 Human
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Alternative names
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
Images
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Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)All lanes : Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) at 1/1000 dilution (Purified)
Lane 1 : Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 170 kDa -
![Immunocytochemistry - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.com/ps/products/32/ab32578/Images/ab32578-9-anti-egfr-phospho-y1173-antibody-e124-immunocytochemistry-a431-human.jpg)
Immunocytochemistry - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with EGF(100ng/ml 5min) cells labeling EGFR with Purified ab32578 at 1:2000 dilution (0.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.com/ps/products/32/ab32578/Images/ab32578-273112-anti-egfr-phospho-y1173-antibody-e124-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab177178 at 1/1300 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.com/ps/products/32/ab32578/Images/ab32578-7-benefits-of-recombinant-antibodies.png)
Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab32578 has been referenced in 29 publications.
- Yen CH et al. Cell detachment ratio on pH-responsive chitosan: A useful biometric for prognostic judgment and drug efficacy assessment in oncology. Carbohydr Polym 261:117911 (2021). PubMed: 33766385
- Zhu Z et al. Eucannabinolide, a novel sesquiterpene lactone, suppresses the growth, metastasis and BCSCS-like traits of TNBC via inactivation of STAT3. Neoplasia 23:36-48 (2021). PubMed: 33217668
- Lin X et al. Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation. Exp Ther Med 21:638 (2021). PubMed: 33968169
- Carrillo-Beltrán D et al. Human Papillomavirus 16 E7 Promotes EGFR/PI3K/AKT1/NRF2 Signaling Pathway Contributing to PIR/NF-?B Activation in Oral Cancer Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32679705
- Ngan HL et al. Erlotinib sensitivity of MAPK1p.D321N mutation in head and neck squamous cell carcinoma. NPJ Genom Med 5:17 (2020). PubMed: 32351709