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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases
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Anti-EGFR (phospho Y992) antibody (ab226873)

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Western blot - Anti-EGFR (phospho Y992) antibody (ab226873)

    Key features and details

    • Rabbit polyclonal to EGFR (phospho Y992)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-EGFR (phospho Y992) antibody
      See all EGFR primary antibodies
    • Description

      Rabbit polyclonal to EGFR (phospho Y992)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human EGFR (internal sequence) (phospho Y992). The exact sequence is proprietary. Conjugated to a carrier protein.
      Database link: P00533

    • Positive control

      • WB: Starved A431 treated with EGF, whole cell extract.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.025% Proclin 300
      Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • EGF
      • Cell Biology
      • Cell Cycle
      • Cell differentiation
      • Cancer
      • Growth factors
      • EGF
      • Cancer
      • Signal transduction
      • Protein phosphorylation
      • Tyrosine kinases
      • Receptor tyrosine kinases
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Growth factor receptors
      • Cancer
      • Tumor biomarkers
      • Receptors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab226873 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Detects a band of approximately 3000 kDa (predicted molecular weight: 134 kDa).

    Target

    • Function

      Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • Tissue specificity

      Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • Involvement in disease

      Lung cancer
      Inflammatory skin and bowel disease, neonatal, 2
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
      Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
    • Cellular localization

      Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
    • Target information above from: UniProt accession P00533 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1956 Human
      • Omim: 131550 Human
      • SwissProt: P00533 Human
      • Unigene: 488293 Human
      • Unigene: 605083 Human
      • Alternative names

        • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
        • Cell growth inhibiting protein 40 antibody
        • Cell proliferation inducing protein 61 antibody
        • EGF R antibody
        • EGFR antibody
        • EGFR_HUMAN antibody
        • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
        • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
        • Epidermal growth factor receptor antibody
        • erb-b2 receptor tyrosine kinase 1 antibody
        • ERBB antibody
        • ERBB1 antibody
        • Errp antibody
        • HER1 antibody
        • mENA antibody
        • NISBD2 antibody
        • Oncogen ERBB antibody
        • PIG61 antibody
        • Proto-oncogene c-ErbB-1 antibody
        • Receptor tyrosine protein kinase ErbB 1 antibody
        • Receptor tyrosine-protein kinase ErbB-1 antibody
        • SA7 antibody
        • Species antigen 7 antibody
        • Urogastrone antibody
        • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
        • wa2 antibody
        • Wa5 antibody
        see all

      Images

      • Western blot - Anti-EGFR (phospho Y992) antibody (ab226873)
        Western blot - Anti-EGFR (phospho Y992) antibody (ab226873)
        All lanes : Anti-EGFR (phospho Y992) antibody (ab226873) at 1/2000 dilution

        Lane 1 : A431 (human epidermoid carcinoma cell line) starved for 16 hours, whole cell extract
        Lane 2 : A431 (human epidermoid carcinoma cell line) starved for 16 hours and then treated with 100 ng/ml EGF for 5 minutes, whole cell extract

        Lysates/proteins at 30 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit IgG

        Predicted band size: 134 kDa



        7.5% SDS-PAGE gel.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab226873? Please let us know so that we can cite the reference in this datasheet.

      ab226873 has not yet been referenced specifically in any publications.

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