Overview

  • Product name

    Anti-EGFR (phospho Y992) antibody [EM-12]
    See all EGFR primary antibodies
  • Description

    Mouse monoclonal [EM-12] to EGFR (phospho Y992)
  • Host species

    Mouse
  • Specificity

    ab81440 recognises EGFR phosphorylated on tyrosine 992.
  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide covering sequence around tyrosine 992 of Human EGFR.

  • Positive control

    • EGF-stimulated A431. A431, CALU-3, Jurkat cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab81440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 134 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificity

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in disease

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • Cellular localization

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • Database links

  • Alternative names

    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Images

  • All lanes : Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440) at 1 µg/ml

    Lane 1 : EGF-treated A431 cell line
    Lane 2 : EGF-treated CALU-3 cell line
    Lane 3 : EGF-treated MCF-7 cell line
    Lane 4 : EGF-treated Jurkat cell line
    Lane 5 : EGF-treated Ramos cell line

    Performed under reducing conditions.

    Predicted band size: 134 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with ab81440 at 1µg/ml and goat anti-mouse-HRP.
  • Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.

References

This product has been referenced in:

  • Pei YF  et al. Silencing of LAMC2 Reverses Epithelial-Mesenchymal Transition and Inhibits Angiogenesis in Cholangiocarcinoma via Inactivation of the Epidermal Growth Factor Receptor Signaling Pathway. Am J Pathol 189:1637-1653 (2019). Read more (PubMed: 31345467) »
  • Dittmann K  et al. New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling. PLoS One 12:e0189087 (2017). Read more (PubMed: 29253018) »
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and test ab81440 in IHC on human tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ICH on human tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting me with this additional information.

I would be happy to create 100% abreview discount codes for each of these applications for you. However each code has to be associated with a single vial. Therefore if you want four codes you would need to purchase four vials of ab81440.

Each 100% Abreview discount code need to be created prior to purchase of the product and referenced when ordering ab81440, submitting the Abreview and purchasing the free primary afterwards.

If you wish to try this product in each of these applications but do not wish to purchase multiple vials of the product, I encourage you to submit Abreviews for the other applications. When you submit Abreviews you are awarded Abpoints which you may collect and use toward future purchases and even Amazon gift cards.

Please let me know how you would like to proceed or if you have further questions.

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Answer

Thank you for contacting Abcam.

I would be glad to create a 100% Abreview discount code for you. Would you be testing ab81440 in human tissues? Further for clarity, do you wish to perform IHC using fluorescence on frozen tissues?

I look forward to your response. Please let me know if you have any questions.

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Answer

Thank you very much for your telephone call yesterday afternoon.

As discussed on the phone, to our knowledge, ab81440has not been tested in ICC-IF or IHC-Fr and regrettably there are no suitably tested alternative mouse monoclonalsavailableto this particular target protein. Therefore, I can offer a discount off a future purchase if you buy ab81440 now, test it in ICC-IF or IHC-Fr and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab81440in ICC-IF or IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab81440 so please wait for my reply before ordering.

2. Purchase ab81440 either by phone, fax, or online (www.abcam.com).

3. Test it inICC-IF or IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab81440 turns out to be unsuitable forICC-IF or IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

With regards to a suitably tested EGFR (phospho Y1173) antibody, we have the following mouse monoclonal available, which has been tested in ICC-IF, IHC-Fr and in human samples. I can recommend to review the online datasheet for further information:

ab24912EGFR (phospho Y1173) antibody [9H2]
https://www.abcam.com/index.html?datasheet=24912 (or use the following: https://www.abcam.com/index.html?datasheet=24912).

I hope this will be helpful. Please let me know if you have any questions about this offer and I would be happy to help you further.

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