Anti-Egr1 antibody (ab182624)
Key features and details
- Rabbit polyclonal to Egr1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Egr1 antibody
See all Egr1 primary antibodies -
Description
Rabbit polyclonal to Egr1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human Egr1 aa 50-350.
Database link: P18146 -
Positive control
- RAW264.7 and NIH 3T3 cell line lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/200 - 1/1000. Predicted molecular weight: 58 kDa.
|
Notes |
---|
WB
1/200 - 1/1000. Predicted molecular weight: 58 kDa. |
Target
-
Function
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. -
Sequence similarities
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- SwissProt: P08046 Mouse
- SwissProt: P08154 Rat
- Unigene: 181959 Mouse
- Unigene: 9096 Rat
-
Alternative names
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
see all
Images
-
All lanes : Anti-Egr1 antibody (ab182624) at 1/400 dilution
Lane 1 : RAW264.7 cell line lysate
Lane 2 : NIH 3T3 cell line lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L HRP conjugated antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 2 minutesGel: 8%SDS-PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab182624 has been referenced in 1 publication.
- Shi W et al. EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells. J Biol Chem 294:6871-6887 (2019). PubMed: 30824540