Product nameAnti-Egr1 antibody
See all Egr1 primary antibodies
Predicted to work with: Mouse, Rat, Human
General notesExpiration date is 1 year from date of opening.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
ab6054 at 10 µg/ml staining human ovarian cancinoma.
This product has been referenced in:
- Zhao J et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). Read more (PubMed: 29393474) »
- Nadra K et al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB ; Mouse . Read more (PubMed: 18559480) »