This fast track antibody is not yet fully characterized. It is subject to these
terms and conditions
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Egr1 antibody
See all Egr1 primary antibodies -
DescriptionRabbit polyclonal to Egr1
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host speciesRabbit
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Species reactivity
Predicted to work with: Mouse, Rat, Human
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Immunogen
Synthetic peptide:
GEQPYEHLTAESFPDI-C
conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1. -
General notesExpiration date is 1 year from date of opening.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IHC-P |
Application notes
Tested in peptide ELISA.
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
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Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- Omim: 128990 Human
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
- SwissProt: P08154 Rat
- Unigene: 326035 Human
see all -
Alternative names
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
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Western blot - Anti-Egr1 antibody (ab6054)Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Secondary
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (ab6054)ab6054 at 10 µg/ml staining human ovarian cancinoma.
References
This product has been referenced in:
- Zhao J et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). Read more (PubMed: 29393474) »
- Nadra K et al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB ; Mouse . Read more (PubMed: 18559480) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"