Overview

  • Product name

    Anti-Egr1 antibody [EPR5014(2)]
    See all Egr1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5014(2)] to Egr1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human, Zebra finch
  • Immunogen

    Synthetic peptide within Human Egr1 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P18146

  • Positive control

    • WB: Transfected 293T cell lysate Flow cyt: NIH/3T3 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 57 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
    • Sequence similarities

      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AT225 antibody
      • Early growth response 1 antibody
      • Early growth response protein 1 antibody
      • EGR 1 antibody
      • EGR-1 antibody
      • EGR1 antibody
      • EGR1_HUMAN antibody
      • G0S30 antibody
      • KROX 24 antibody
      • KROX24 antibody
      • Nerve growth factor-induced clone A antibody
      • Nerve growth factor-induced protein A antibody
      • NGFI-A antibody
      • NGFIA antibody
      • TIS8 antibody
      • Transcription factor ETR103 antibody
      • Transcription factor Zif268 antibody
      • ZIF 268 antibody
      • ZIF268 antibody
      • Zinc finger protein 225 antibody
      • Zinc finger protein Krox-24 antibody
      • ZNF225 antibody
      see all

    Images

    • All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/1000 dilution

      Lane 1 : untreated 293 cell lysate
      Lane 2 : 293 cell lysate treated with 20% FBS

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • ab133695 (red), at 1/10, staining Egr1 in permeabilized NIH/3T3 cells treated with 20% FBS using flow cytometric analysis. The green curve corresponds to a Rabbit IgG (negative control).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Liu L  et al. Hippocampal Mechanisms Underlying Impairment in Spatial Learning Long After Establishment of Noise-Induced Hearing Loss in CBA Mice. Front Syst Neurosci 12:35 (2018). Read more (PubMed: 30087600) »
    • Mao G  et al. Long term exposure to ?-rays induces radioresistance and enhances the migration ability of bladder cancer cells. Mol Med Rep 18:5834-5840 (2018). Read more (PubMed: 30365074) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (7.5% Tris Glycine)
    Sample
    Zebra finch Cell lysate - whole cell (Hek cells overexpressing zebra finch EGR1)
    Specification
    Hek cells overexpressing zebra finch EGR1
    Treatment
    MG132
    Blocking step
    Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 28 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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