Product nameAnti-Egr1 antibody [EPR5014(2)]
See all Egr1 primary antibodies
DescriptionRabbit monoclonal [EPR5014(2)] to Egr1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Human, Zebra finch
Synthetic peptide within Human Egr1 aa 1-100 (internal sequence). The exact sequence is proprietary.
Database link: P18146
- WB: Transfected 293T cell lysate Flow cyt: NIH/3T3 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.60 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 57 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranscriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/1000 dilution
Lane 1 : untreated 293 cell lysate
Lane 2 : 293 cell lysate treated with 20% FBS
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab133695 (red), at 1/10, staining Egr1 in permeabilized NIH/3T3 cells treated with 20% FBS using flow cytometric analysis. The green curve corresponds to a Rabbit IgG (negative control).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Liu L et al. Hippocampal Mechanisms Underlying Impairment in Spatial Learning Long After Establishment of Noise-Induced Hearing Loss in CBA Mice. Front Syst Neurosci 12:35 (2018). Read more (PubMed: 30087600) »
- Mao G et al. Long term exposure to ?-rays induces radioresistance and enhances the migration ability of bladder cancer cells. Mol Med Rep 18:5834-5840 (2018). Read more (PubMed: 30365074) »