• Product name

    Anti-Egr1 antibody [mAbcam55160]
    See all Egr1 primary antibodies
  • Description

    Mouse monoclonal [mAbcam55160] to Egr1
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Egr1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab56196.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell Lysate; HeLa Nuclear lysate - TPA stimulated 2 hr
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab55160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 55, 85 kDa (predicted molecular weight: 58 kDa).
IP Use a concentration of 5 µg/ml.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.



  • Function

    Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
  • Sequence similarities

    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • AT225 antibody
    • Early growth response 1 antibody
    • Early growth response protein 1 antibody
    • EGR 1 antibody
    • EGR-1 antibody
    • EGR1 antibody
    • EGR1_HUMAN antibody
    • G0S30 antibody
    • KROX 24 antibody
    • KROX24 antibody
    • Nerve growth factor-induced clone A antibody
    • Nerve growth factor-induced protein A antibody
    • NGFI-A antibody
    • NGFIA antibody
    • TIS8 antibody
    • Transcription factor ETR103 antibody
    • Transcription factor Zif268 antibody
    • ZIF 268 antibody
    • ZIF268 antibody
    • Zinc finger protein 225 antibody
    • Zinc finger protein Krox-24 antibody
    • ZNF225 antibody
    see all


  • All lanes : Anti-Egr1 antibody [mAbcam55160] (ab55160) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 55,85 kDa
    why is the actual band size different from the predicted?

  • Egr1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Egr1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab55160.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 55kDa; Egr1

  • ab55160 (1/250) staining Egr1 in paraffin-embedded Human placenta tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  The secondary antibody was goat anti rabbit/mouse conjugated to HRP.  For further experimental details please refer to abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab55160 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55160, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:

  • Zhang YM  et al. Autophagy-related gene LRRK2 is likely a susceptibility gene for systemic lupus erythematosus in northern Han Chinese. Oncotarget 8:13754-13761 (2017). Read more (PubMed: 28099919) »
  • Huang Q  et al. HTLV-1 Tax upregulates early growth response protein 1 through nuclear factor-?B signaling. Oncotarget 8:51123-51133 (2017). Read more (PubMed: 28881635) »
See all 7 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

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Submitted Mar 25 2010

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