The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100. Predicted molecular weight: 57 kDa.
1/10 - 1/50.
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Flow cytometry analysis of WiDr cells labeling Egr1 with ab174509 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of HeLa cells labeling Egr1 with ab174509 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-Egr1 antibody - N-terminal (ab174509)
Anti-Egr1 antibody - N-terminal (ab174509) at 1/50 dilution + MDA-MB231 cell lysate at 35 µg
Minich RR et al. Early growth response protein 1 regulates promoter activity of a-plasma membrane calcium ATPase 2, a major calcium pump in the brain and auditory system. BMC Mol Biol18:14 (2017).
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