Key features and details
- Goat polyclonal to EGR2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EGR2 antibody
See all EGR2 primary antibodies
DescriptionGoat polyclonal to EGR2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
- WB: HepG2 whole cell lysate (ab7900). IHC-P: Human colon tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab63943 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab63943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 5 µg/ml.|
FunctionSequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
Involvement in diseaseDefects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
Sequence similaritiesBelongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
modificationsUbiquitinated by WWP2 leading to proteasomal degradation.
- Information by UniProt
- AT591 antibody
- CMT1D antibody
- CMT4E antibody
Anti-EGR2 antibody (ab63943) at 2 µg/ml + HepG2 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Incubated with the primary antibody for 1 hour.
ab63943 at 3µg/ml staining EGR2 in human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP-staining procedure was used for detection.
ab63943 has been referenced in 1 publication.
- Wilcox MB et al. Characterising cellular and molecular features of human peripheral nerve degeneration. Acta Neuropathol Commun 8:51 (2020). PubMed: 32303273