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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-EGR2 antibody [EPR4004] (ab108399)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (11)

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Western blot - Anti-EGR2 antibody [EPR4004] (ab108399)
  • Anti-EGR2 antibody [EPR4004] (ab108399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4004] to EGR2
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-EGR2 antibody [EPR4004]
    See all EGR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4004] to EGR2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • LnCaP, HepG2, MCF7, and SH SY5Y cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4004
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other

Associated products

  • Alternative Versions

    • Anti-EGR2 antibody [EPR4004] - BSA and Azide free (ab232368)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)
    • LNCaP nuclear extract lysate (ab14857)
    • MCF7 nuclear extract lysate (ab14860)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Rat
IP
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
    • Involvement in disease

      Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    • Sequence similarities

      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Ubiquitinated by WWP2 leading to proteasomal degradation.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P11161 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1959 Human
      • Entrez Gene: 13654 Mouse
      • Entrez Gene: 114090 Rat
      • GenBank: BC035625 Human
      • Omim: 129010 Human
      • SwissProt: P11161 Human
      • SwissProt: P08152 Mouse
      • SwissProt: P51774 Rat
      • Unigene: 1395 Human
      • Unigene: 290421 Mouse
      • Unigene: 89235 Rat
      see all
    • Alternative names

      • AT591 antibody
      • CMT1D antibody
      • CMT4E antibody
      • DKFZp686J1957 antibody
      • E3 SUMO-protein ligase EGR2 antibody
      • Early growth response 2 antibody
      • Early growth response protein 2 antibody
      • EGR-2 antibody
      • egr2 antibody
      • EGR2_HUMAN antibody
      • FLJ14547 antibody
      • KROX 20 Drosophila homolog antibody
      • Krox 20 homolog Drosophila antibody
      • KROX-20, Drosophila, homolog (early growth response-2) antibody
      • KROX20 antibody
      • Krox20 protein antibody
      • Zinc finger protein Krox-20 antibody
      see all

    Images

    • Western blot - Anti-EGR2 antibody [EPR4004] (ab108399)
      Western blot - Anti-EGR2 antibody [EPR4004] (ab108399)
      All lanes : Anti-EGR2 antibody [EPR4004] (ab108399) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : SH SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Anti-EGR2 antibody [EPR4004] (ab108399)
      Anti-EGR2 antibody [EPR4004] (ab108399)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (11)

    Publishing research using ab108399? Please let us know so that we can cite the reference in this datasheet.

    ab108399 has been referenced in 11 publications.

    • Li Z  et al. MicroRNA-124 Overexpression in Schwann Cells Promotes Schwann Cell-Astrocyte Integration and Inhibits Glial Scar Formation Ability. Front Cell Neurosci 14:144 (2020). PubMed: 32714149
    • Liu F  et al. Increased T-helper 17 cell differentiation mediated by exosome-mediated microRNA-451 redistribution in gastric cancer infiltrated T cells. Cancer Sci 109:65-73 (2018). WB . PubMed: 29059496
    • Miyamoto Y  et al. BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice. Sci Adv 4:eaar4471 (2018). PubMed: 29740613
    • Niu RN  et al. Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway. Biochimie 149:62-70 (2018). WB ; Rat . PubMed: 29580816
    • Fan Y  et al. MiR-377 Regulates Inflammation and Angiogenesis in Rats After Cerebral Ischemic Injury. J Cell Biochem 119:327-337 (2018). PubMed: 28569430
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-EGR2 antibody [EPR4004]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2016

    Western blot abreview for Anti-EGR2 antibody [EPR4004]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 03 2016

    Western blot abreview for Anti-EGR2 antibody [EPR4004]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Specification
    U2OS cells
    Treatment
    50 pM TGF-beta time course
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 03 2014

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