Overview

  • Product name

  • Description

    Rabbit polyclonal to EGR4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human EGR4 (internal sequence).
    Database link: Q05215

  • Positive control

    • Human fetal brain tissue lysate; Human lung cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab198197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 51 kDa.

Target

  • Relevance

    EGR4(Early Growth Response protein 4) is a zinc-finger transcription factor that activates the transcription of genes whose products are required for mitogenesis and differentiation. EGR4 is closely related to nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR4 functionally cooperate with NFAT proteins and induce expression of IL2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AT133 antibody
    • Early growth response protein 4 antibody
    • Nerve growth factor induced clone C antibody
    • NGFI C antibody
    • NGFIC antibody
    • PAT133 antibody
    see all

Images

  • Anti-EGR4 antibody (ab198197) at 1/400 dilution + Human fetal brain tissue lysate at 40 µg

    Predicted band size: 51 kDa


    Exposure time: 35 seconds


    8% SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling EGR4 with ab198197 at a 1/25 dilution.

References

This product has been referenced in:

  • Niu RN  et al. Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway. Biochimie 149:62-70 (2018). WB ; Rat . Read more (PubMed: 29580816) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Ferret Tissue lysate - whole (cerebral cortex)
Gel Running Conditions
Reduced Denaturing (NuPage 10% Bis-Tris gel)
Loading amount
40 µg
Specification
cerebral cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 12 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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