Product nameAnti-EGR4 antibody
See all EGR4 primary antibodies
DescriptionRabbit polyclonal to EGR4
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Drosophila melanogaster, Zebrafish
- Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Peptide ELISA titre: 1/312500.
|WB||Use a concentration of 0.0625 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceEGR4(Early Growth Response protein 4) is a zinc-finger transcription factor that activates the transcription of genes whose products are required for mitogenesis and differentiation. EGR4 is closely related to nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR4 functionally cooperate with NFAT proteins and induce expression of IL2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
- AT133 antibody
- Early growth response protein 4 antibody
- Nerve growth factor induced clone C antibody
Anti-EGR4 antibody (ab50636) at 0.0625 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.
Predicted band size: 51 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
The primary antibody was diluted in 5% skim milk/PBS buffer.
ab50636 has not yet been referenced specifically in any publications.