Anti-EHBP1L1 antibody (ab122557)
Key features and details
- Rabbit polyclonal to EHBP1L1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHBP1L1 antibody -
Description
Rabbit polyclonal to EHBP1L1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: IEVGLLGVLG IETGAAEGAI LGTQEIASRD SGVPGLEADT TGIQVKEVGG SEVPEIATGT AETEILGTQE IASRSSGVPG LESEVAGAQE TEVGGSGISG, corresponding to amino acids of Human EHBP1L1.
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Positive control
- Human stomach tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
| IHC-P | 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 254102 Human
- SwissProt: Q8N3D4 Human
- Unigene: 502867 Human
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Alternative names
- DKFZp762C186 antibody
- EH domain-binding protein 1-like protein 1 antibody
- EH1L1_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EHBP1L1 antibody (ab122557)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122557 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (ab122557)ab122557, at 1/2500 dilution, staining EHBP1L1 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References (0)
ab122557 has not yet been referenced specifically in any publications.
