Anti-EHD1 antibody [EPR4954] (ab109311)
- Datasheet
- Specific References (4)
- Protocols
Overview
-
Product nameAnti-EHD1 antibody [EPR4954]
See all EHD1 primary antibodies -
DescriptionRabbit monoclonal [EPR4954] to EHD1
-
Host speciesRabbit
-
Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human EHD1. The exact sequence is proprietary.
-
Positive control
- WB: HepG2, Human testis, SW480, SK-OV-3 and HeLa lysates IHC-P: Human kidney and tonsil tissues ICC/IF: HeLa cells FC: HeLa cells
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR4954
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/30. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat to 98°C, allow to cool for 10-20 minutes) | |
| ICC/IF | 1/100 - 1/250. |
Target
-
FunctionActs in early endocytic membrane fusion and membrane trafficking of recycling endosomes.
-
Tissue specificityHighly expressed in testis.
-
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 EH domain. -
DomainThe EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
-
Cellular localizationCell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures.
- Information by UniProt
-
Database links
- Entrez Gene: 10938 Human
- Entrez Gene: 13660 Mouse
- Entrez Gene: 293692 Rat
- Omim: 605888 Human
- SwissProt: Q9H4M9 Human
- SwissProt: Q9WVK4 Mouse
- SwissProt: Q641Z6 Rat
- Unigene: 523774 Human
see all -
Alternative names
- CDABP0131 antibody
- EH domain containing 1 antibody
- EH domain-containing protein 1 antibody
see all
Images
-
![Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)](https://www.abcam.com/ps/products/109/ab109311/Images/EHD1-Primary-antibodies-ab109311-1.jpg)
Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : Human testis lysates
Lane 3 : SW480 cell lysates
Lane 4 : SK-OV-3 cell lysates
Lane 5 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Observed band size: 60 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)](https://www.abcam.com/ps/products/109/ab109311/Images/EHD1-Primary-antibodies-ab109311-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using ab109311 at a 1/100 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4954] (ab109311)](https://www.abcam.com/ps/products/109/ab109311/Images/EHD1-Primary-antibodies-ab109311-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4954] (ab109311)Immunofluorescent staining of EHD1 in HeLa cells, using ab109311 at a 1/100 dilution. -
![Flow Cytometry - Anti-EHD1 antibody [EPR4954] (ab109311)](https://www.abcam.com/ps/products/109/ab109311/Images/ab109311-271361-ab109311FC1.jpg)
Flow Cytometry - Anti-EHD1 antibody [EPR4954] (ab109311)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling EHD1 with unpurified ab109311 at 1/30 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)](https://www.abcam.com/ps/products/109/ab109311/Images/EHD1-Primary-antibodies-ab109311-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using ab109311 at a 1/100 dilution.
Protocols
References
This product has been referenced in:
- Meng Q et al. Mammalian Eps15 homology domain 1 promotes metastasis in non-small cell lung cancer by inducing epithelial-mesenchymal transition. Oncotarget 8:22433-22442 (2017). IHC-P ; Human . Read more (PubMed: 27531895) »
- Yeow I et al. EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress. Curr Biol 27:2951-2962.e5 (2017). Read more (PubMed: 28943089) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"