Recombinant Anti-EHD1 antibody [EPR4954] (ab109311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4954] to EHD1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EHD1 antibody [EPR4954]
See all EHD1 primary antibodies -
Description
Rabbit monoclonal [EPR4954] to EHD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human EHD1. The exact sequence is proprietary.
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Positive control
- WB: HepG2, Human testis, SW480, SK-OV-3, Human brain, HeLa, Mouse brain, and Rat brain lysates. IHC-P: human thyroid carcinoma, kidney and tonsil, mouse lung, and rat lung tissues. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4954 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). | |
IP | 1/10 - 1/100. | |
IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat to 98°C, allow to cool for 10-20 minutes) 1:120 in human tissues / 1:1600 in mouse and rat tissues |
Target
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Function
Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 EH domain. -
Domain
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. -
Cellular localization
Cell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures. - Information by UniProt
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Database links
- Entrez Gene: 10938 Human
- Entrez Gene: 13660 Mouse
- Entrez Gene: 293692 Rat
- Omim: 605888 Human
- SwissProt: Q9H4M9 Human
- SwissProt: Q9WVK4 Mouse
- SwissProt: Q641Z6 Rat
- Unigene: 523774 Human
see all -
Alternative names
- CDABP0131 antibody
- EH domain containing 1 antibody
- EH domain-containing protein 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling EHD1 with purified ab109311 at 1/120 dilution (2.85 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab109311 (purified ) at 1/30 dilution (2ug) immunoprecipitating EHD1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab109311 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109311 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution ((unpurified))
Lane 1 : HepG2 cell lysates
Lane 2 : Human testis lysates
Lane 3 : SW480 cell lysates
Lane 4 : SK-OV-3 cell lysates
Lane 5 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using unpurified ab109311 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using unpurified ab109311 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (15)
ab109311 has been referenced in 15 publications.
- Jones T et al. Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and fission. PLoS One 15:e0239657 (2020). PubMed: 32966336
- Kamerkar SC et al. A Screen for Membrane Fission Catalysts Identifies the ATPase EHD1. Biochemistry 58:65-71 (2019). PubMed: 30403133
- Insinna C et al. Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Nat Commun 10:428 (2019). PubMed: 30683896
- Wang T et al. Mammalian Eps15 homology domain 1 potentiates angiogenesis of non-small cell lung cancer by regulating ß2AR signaling. J Exp Clin Cancer Res 38:174 (2019). PubMed: 31023336
- Liu Y et al. Eps15 homology domain 1 promotes the evolution of papillary thyroid cancer by regulating endocytotic recycling of epidermal growth factor receptor. Oncol Lett 16:4263-4270 (2018). PubMed: 30214560