Overview

  • Product name

    Anti-EHD1 antibody [EPR4954] (Alexa Fluor® 647)
    See all EHD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4954] to EHD1 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EHD1. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/625.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Contains 1 EF-hand domain.
    Contains 1 EH domain.
  • Domain

    The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
  • Cellular localization

    Cell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDABP0131 antibody
    • EH domain containing 1 antibody
    • EH domain-containing protein 1 antibody
    • EHD1 antibody
    • EHD1_HUMAN antibody
    • FLJ42622 antibody
    • FLJ44618 antibody
    • H PAST antibody
    • hPAST1 antibody
    • PAST antibody
    • PAST homolog 1 antibody
    • PAST1 antibody
    • Testilin antibody
    see all

Images

  • ab201354 staining EHD1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201354 at a 1/625 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

References

ab201354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201354.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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