Product nameAnti-EHD1 antibody [EPR4955]
See all EHD1 primary antibodies
DescriptionRabbit monoclonal [EPR4955] to EHD1
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EHD1 aa 350-450 (internal sequence). The exact sequence is proprietary.
- HepG2, HeLa and JAR cell lysates; HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab109747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 61 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionActs in early endocytic membrane fusion and membrane trafficking of recycling endosomes.
Tissue specificityHighly expressed in testis.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 EH domain.
DomainThe EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Cellular localizationCell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures.
- Information by UniProt
- CDABP0131 antibody
- EH domain containing 1 antibody
- EH domain-containing protein 1 antibody
Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.
This product has been referenced in:
- Vidal-Quadras M et al. Endocytic turnover of Rab8 controls cell polarization. J Cell Sci 130:1147-1157 (2017). Read more (PubMed: 28137756) »
- Matsudaira T et al. Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells. Nat Commun 8:1246 (2017). Read more (PubMed: 29093443) »