Key features and details
- Rabbit polyclonal to EHD2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-EHD2 antibody
See all EHD2 primary antibodies
DescriptionRabbit polyclonal to EHD2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- WB: HeLa whole cell lysate (ab150035); Mouse liver and kidney lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 62, 47 kDa (predicted molecular weight: 61, 46 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionPlays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
Tissue specificityHighly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 EH domain.
DomainThe EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Cellular localizationCell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin.
- Information by UniProt
- EH domain containing 2 antibody
- EH domain containing 3 antibody
- EH domain containing protein 2 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EHD2 (green) using ab222888 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
All lanes : Anti-EHD2 antibody (ab222888) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 46 kDa
Observed band size: 47,62 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for EHD2 using ab222888 at 1/100 dilution in immunohistochemical analysis.
ab222888 has not yet been referenced specifically in any publications.