Recombinant Anti-EHD2 antibody [EPR9821] (ab154784)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9821] to EHD2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EHD2 antibody [EPR9821]
See all EHD2 primary antibodies -
Description
Rabbit monoclonal [EPR9821] to EHD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal heart, A549 and HeLa lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9821 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP. -
Tissue specificity
Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 EH domain. -
Domain
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. -
Cellular localization
Cell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. - Information by UniProt
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Database links
- Entrez Gene: 30846 Human
- Entrez Gene: 259300 Mouse
- Entrez Gene: 361512 Rat
- Omim: 605890 Human
- SwissProt: Q9NZN4 Human
- SwissProt: Q8BH64 Mouse
- SwissProt: Q4V8H8 Rat
- Unigene: 631554 Human
see all -
Alternative names
- EH domain containing 2 antibody
- EH domain containing 3 antibody
- EH domain containing protein 2 antibody
see all
Images
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All lanes : Anti-EHD2 antibody [EPR9821] (ab154784) at 1/1000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa -
Immunofluorescent analysis of HeLa cells labeling EHD2 with ab154784 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154784 has been referenced in 2 publications.
- Morén B et al. EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes. Mol Biol Cell 30:1147-1159 (2019). PubMed: 30811273
- Li Z et al. A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Sci Adv 2:e1601470 (2016). PubMed: 28028537