Overview

  • Product name
    Anti-EHD2 antibody [EPR9821]
    See all EHD2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9821] to EHD2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human EHD2 (UniProt Q9NZN4).

  • Positive control
    • Fetal heart, A549 and HeLa lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
    • Tissue specificity
      Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
    • Sequence similarities
      Contains 1 EF-hand domain.
      Contains 1 EH domain.
    • Domain
      The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
    • Cellular localization
      Cell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin.
    • Information by UniProt
    • Database links
    • Alternative names
      • EH domain containing 2 antibody
      • EH domain containing 3 antibody
      • EH domain containing protein 2 antibody
      • EH domain-containing protein 2 antibody
      • EHD 2 antibody
      • Ehd2 antibody
      • EHD2_HUMAN antibody
      • EHD3 antibody
      • FLJ96617 antibody
      • PAST 2 antibody
      • PAST homolog 2 antibody
      • PAST2 antibody
      see all

    Images

    • All lanes : Anti-EHD2 antibody [EPR9821] (ab154784) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 61 kDa

    • Immunofluorescent analysis of HeLa cells labeling EHD2 with ab154784 at 1/250 dilution.

    References

    This product has been referenced in:
    • Li Z  et al. A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Sci Adv 2:e1601470 (2016). Read more (PubMed: 28028537) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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