Overview

  • Product name

  • Description

    Rabbit polyclonal to EHD4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 52-315 of Human EHD4 (Uniprot ID: Q9H223).

  • Positive control

    • 293T, A431, HeLa and HepG2 whole cell lysates; Human BT483 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Relevance

    EHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway.
  • Cellular localization

    Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.
  • Database links

  • Alternative names

    • EH domain containing 4 antibody
    • EH domain containing protein 4 antibody
    • EH domain-containing protein 4 antibody
    • EH domaincontaining protein 4 antibody
    • EH-domain containing 4 antibody
    • EHdomain containing 4 antibody
    • EHdomain containing4 antibody
    • Hepatocellular carcinoma associated protein 10/11 antibody
    • hepatocellular carcinoma-associated protein 10/11 antibody
    • hepatocellular carcinoma-associated protein HCA11 antibody
    • HGNC:3245 antibody
    • Ortholog of rat pincher antibody
    • PAST homolog 4 antibody
    • PAST4 antibody
    see all

Images

  • All lanes : Anti-EHD4 antibody (ab153892) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue labeling EHD4 with ab153892 at 1/500 dilution.

References

This product has been referenced in:

  • Feng J  et al. Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20. J Virol 92:N/A (2018). Read more (PubMed: 29695422) »
  • Dutta S  et al. Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology. Cancer Res 76:418-28 (2016). WB ; Human . Read more (PubMed: 26560516) »
See all 2 Publications for this product

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