Key features and details
- Rabbit polyclonal to EHD4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EHD4 antibody
See all EHD4 primary antibodies
DescriptionRabbit polyclonal to EHD4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 52-315 of Human EHD4 (Uniprot ID: Q9H223).
- 293T, A431, HeLa and HepG2 whole cell lysates; Human BT483 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/1000.|
RelevanceEHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway.
Cellular localizationEarly endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.
- EH domain containing 4 antibody
- EH domain containing protein 4 antibody
- EH domain-containing protein 4 antibody
All lanes : Anti-EHD4 antibody (ab153892) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue labeling EHD4 with ab153892 at 1/500 dilution.
ab153892 has been referenced in 2 publications.
- Feng J et al. Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20. J Virol 92:N/A (2018). PubMed: 29695422
- Dutta S et al. Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology. Cancer Res 76:418-28 (2016). WB ; Human . PubMed: 26560516