Key features and details
- Rabbit polyclonal to EHF
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EHF antibody
See all EHF primary antibodies
DescriptionRabbit polyclonal to EHF
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee
- WB: TD47D whole cell lysates; ICC: A431 whole cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 34 kDa.|
FunctionTranscriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
Tissue specificityExpressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
DomainThe PNT domain acts as a transcriptional activator.
- Information by UniProt
- EHF antibody
- EHF_HUMAN antibody
- Epithelium-specific Ets transcription factor 3 antibody
Anti-EHF antibody (ab220113) at 0.4 µg/ml + TD47D (human breast invasive ductal carcinoma cell line) whole cell lysate
Predicted band size: 34 kDa
Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling EHF (green) in the nucleoplasm and Golgi apparatus with ab220113 at 2 μg/ml.
ab220113 has not yet been referenced specifically in any publications.