Overview

  • Product name
  • Description
    Rabbit polyclonal to EHF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: PFQEFDINGE HLCSMSLQEF TRAAGTAGQL LYSNLQHLKW NGQCSSDLFQ, corresponding to N terminal amino acids 74-123 of Human EHF

Properties

Applications

Our Abpromise guarantee covers the use of ab24337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
  • Tissue specificity
    Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Domain
    The PNT domain acts as a transcriptional activator.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EHF antibody
    • EHF_HUMAN antibody
    • Epithelium-specific Ets transcription factor 3 antibody
    • ESE-3 antibody
    • ESE3 antibody
    • ESE3 transcription factor antibody
    • ESE3B antibody
    • ESEJ antibody
    • Ets domain transcription factor antibody
    • ETS domain-containing transcription factor antibody
    • ETS homologous factor antibody
    • hEHF antibody
    see all

Images

  • Anti-EHF antibody (ab24337) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000-1/100000

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.



    ab24337 was used at a dilution of 1.25 ug/ml in 5% skim milk / PBS buffer, with a secondary HRP conjugated anti-Rabbit IgG. Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EHF with ab24337 at 8µg/ml. Renal tubule epithelial cells labelled are indicated with arrows. Magnification: X400.

References

ab24337 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab24337. I can recommend to start with the western blot first. I would also appreciate if you can confirm some further details: 1. I would appreciate if you are able to provide more details of how overexpressing LnCAP cells are prepared, the transfection etc. 2. There are inherent difficulties with antibody detection of recombinant proteins that I can recommend considering. This may be a biological artifact rather than a problem with the antibody. Could you confirm that the protein transfected is full length and includes the immunogen sequence? Also, if the recombinant EHF is tagged, it is possible that the tag is preventing access to the antibody. Could you confirm at which region of the protein the tag has been placed? 3. Could you confirm the species from which the recombinant EHF gene was taken? 4. Could you confirm how the stability of transfection has been assessed? 5. I can recommend that due to the points described above, it is important to use an endogenous positive control sample. For example, prostate and lung are known to have high expression and lysate from one of these tissues should be suitable. 6. In order to increase the signal, I can suggest to try increasing the antibody concentration to 2 - 3 µg/ml. 7. Could you confirm that the transfer of protein to the membrane and quality of the sample have been assessed using a loading control? 8. Is the current vial of secondary antibody working with other primary antibodies? In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note or free of charge replacement. Regarding the IHC, I am sorry to confirm that the attachment did not come through. I would appreciate if you could try sending this again. However, as discussed above, I would recommend to review the WB first. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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