Product nameAnti-EHF antibody
See all EHF primary antibodies
DescriptionRabbit polyclonal to EHF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab24337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionTranscriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
Tissue specificityExpressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
DomainThe PNT domain acts as a transcriptional activator.
- Information by UniProt
- EHF antibody
- EHF_HUMAN antibody
- Epithelium-specific Ets transcription factor 3 antibody
Anti-EHF antibody (ab24337) at 1.25 µg/ml + Jurkat cell lysate
HRP conjugated anti-Rabbit IgG at 1/50000-1/100000
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
ab24337 was used at a dilution of 1.25 ug/ml in 5% skim milk / PBS buffer, with a secondary HRP conjugated anti-Rabbit IgG. Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EHF with ab24337 at 8µg/ml. Renal tubule epithelial cells labelled are indicated with arrows. Magnification: X400.
ab24337 has not yet been referenced specifically in any publications.