Anti-EHF antibody (ab272671)
Key features and details
- Rabbit polyclonal to EHF
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHF antibody
See all EHF primary antibodies -
Description
Rabbit polyclonal to EHF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Chimpanzee -
Immunogen
Recombinant fragment corresponding to Human EHF aa 1-100.
Database link: Q9NZC4 -
Positive control
- IHC-P: Human salivary and breast tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene. -
Tissue specificity
Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Domain
The PNT domain acts as a transcriptional activator. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 739336 Chimpanzee
- Entrez Gene: 505422 Cow
- Entrez Gene: 26298 Human
- Entrez Gene: 13661 Mouse
- Omim: 605439 Human
- SwissProt: A2T737 Chimpanzee
- SwissProt: Q32LN0 Cow
- SwissProt: Q9NZC4 Human
see all -
Alternative names
- EHF antibody
- EHF_HUMAN antibody
- Epithelium-specific Ets transcription factor 3 antibody
see all
Images
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Paraffin-embedded formalin-fixed human salivary tissue stained for EHF using ab272671 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded formalin-fixed human breast tissue stained for EHF using ab272671 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded formalin-fixed human liver tissue stained for EHF using ab272671 at 1/200 dilution in immunohistochemical analysis. Shows no expression as expected.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab272671 has been referenced in 1 publication.
- Scimeca M et al. The ETS Homologous Factor (EHF) Represents a Useful Immunohistochemical Marker for Predicting Prostate Cancer Metastasis. Diagnostics (Basel) 12:N/A (2022). PubMed: 35453848