Anti-EHHADH antibody (ab136059)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-EHHADH antibody
See all EHHADH primary antibodies -
Description
Rabbit polyclonal to EHHADH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide derived from an internal region of Human EHHADH.
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Positive control
- HepG2 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136059 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab136059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 79 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Liver and kidney. Lower amounts seen in the brain. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
Post-translational
modificationsAcetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 1962 Human
- Entrez Gene: 171142 Rat
- Omim: 607037 Human
- SwissProt: Q08426 Human
- SwissProt: P07896 Rat
- Unigene: 429879 Human
- Unigene: 3671 Rat
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Alternative names
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
Images
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Western blot - Anti-EHHADH antibody (ab136059)Anti-EHHADH antibody (ab136059) at 1/500 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 79 kDa
Datasheets and documents
References
ab136059 has not yet been referenced specifically in any publications.
