Anti-EHHADH antibody (ab136059)
Key features and details
- Rabbit polyclonal to EHHADH
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EHHADH antibody
See all EHHADH primary antibodies -
Description
Rabbit polyclonal to EHHADH -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide derived from an internal region of Human EHHADH.
-
Positive control
- HepG2 cell lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136059 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab136059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 79 kDa. |
Target
-
Tissue specificity
Liver and kidney. Lower amounts seen in the brain. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
Post-translational
modificationsAcetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. -
Cellular localization
Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 1962 Human
- Entrez Gene: 171142 Rat
- Omim: 607037 Human
- SwissProt: Q08426 Human
- SwissProt: P07896 Rat
- Unigene: 429879 Human
- Unigene: 3671 Rat
-
Alternative names
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
Images
-
Western blot - Anti-EHHADH antibody (ab136059)Anti-EHHADH antibody (ab136059) at 1/500 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 79 kDa
Datasheets and documents
References (0)
ab136059 has not yet been referenced specifically in any publications.
