Anti-EHM2 antibody (ab77484)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.

Overview

  • Product name

  • Description

    Goat polyclonal to EHM2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Goat
  • Specificity

    Expected to recognise both reported isoforms (NP_060894.2; NP_061987.3).
  • Species reactivity


    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-HNYQPQYHPNIHPSQ

    , corresponding to internal sequence amino acids 445-459 of Human EHM2 (NP_060894.2; NP_061987.3).

  • Positive control

    • Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

  • Application notes
    Predicted molecular weight for endogenous protein: 100 kDa.
    Preliminary experiments gave an approx 48kDa band in Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates after 0.3µg/ml antibody staining. The 48kDa band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References

    This product has been referenced in:

    • Zihni C  et al. An apical MRCK-driven morphogenetic pathway controls epithelial polarity. Nat Cell Biol 19:1049-1060 (2017). Read more (PubMed: 28825699) »
    • Nakajima H & Tanoue T Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF. J Cell Biol 195:245-61 (2011). Read more (PubMed: 22006950) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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