Key features and details
- Mouse monoclonal [B0422] to EHMT1/GLP
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-EHMT1/GLP antibody [B0422]
See all EHMT1/GLP primary antibodies
DescriptionMouse monoclonal [B0422] to EHMT1/GLP
Tested Applications & Species
Application Species ICC/IFMouse
Recombinant fragment corresponding to Mouse EHMT1/GLP aa 134-234.
Database link: Q5DW34
- WT mouse ES cell
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityTissue culture supernatant
ChIP Related Products
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
Contains 8 ANK repeats.
Contains 1 pre-SET domain.
Contains 1 SET domain.
DomainThe ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
The SET domain mediates interaction with WIZ.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome. Associates with euchromatic regions.
- Information by UniProt
- bA188C12.1 antibody
- DKFZp667M072 antibody
- EHMT 1 antibody
ab41969 has been referenced in 18 publications.
- Frega M et al. Distinct Pathogenic Genes Causing Intellectual Disability and Autism Exhibit a Common Neuronal Network Hyperactivity Phenotype. Cell Rep 30:173-186.e6 (2020). PubMed: 31914384
- Karl M et al. Recruitment of Histone Methyltransferase Ehmt1 to Foxp3 TSDR Counteracts Differentiation of Induced Regulatory T Cells. J Mol Biol 431:3606-3625 (2019). PubMed: 31362003
- Frega M et al. Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling. Nat Commun 10:4928 (2019). PubMed: 31666522
- Li H et al. Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. Nat Commun 9:1420 (2018). PubMed: 29650963
- Yang Y et al. Euchromatic histone lysine methyltransferase 1 regulates cancer development in human gastric cancer by regulating E-cadherin. Oncol Lett 15:9480-9486 (2018). PubMed: 29844834