Overview

  • Product name
    Anti-EHMT1/GLP antibody [B0422]
    See all EHMT1/GLP primary antibodies
  • Description
    Mouse monoclonal [B0422] to EHMT1/GLP
  • Host species
    Mouse
  • Tested applications
    Suitable for: ChIP, ICC/IF, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Mouse EHMT1/GLP aa 134-234.
    Database link: Q5DW34

  • Positive control
    • WT mouse ES cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes
    ab41969 was purified by Ammonium sulfate fractionation
  • Clonality
    Monoclonal
  • Clone number
    B0422
  • Myeloma
    NS1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 18066052
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa.
ELISA Use a concentration of 0.1 µg/ml.
IP Use at an assay dependent dilution.

Target

  • Function
    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family.
    Contains 8 ANK repeats.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain
    The ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
    The SET domain mediates interaction with WIZ.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome. Associates with euchromatic regions.
  • Information by UniProt
  • Database links
    • Alternative names
      • bA188C12.1 antibody
      • DKFZp667M072 antibody
      • EHMT 1 antibody
      • EHMT1 antibody
      • EHMT1_HUMAN antibody
      • Eu-HMTase1 antibody
      • Euchromatic histone lysine N methyltransferase 1 antibody
      • Euchromatic histone methyltransferase 1 antibody
      • Euchromatic histone-lysine N-methyltransferase 1 antibody
      • EUHMTASE1 antibody
      • FLJ12879 antibody
      • FP13812 antibody
      • G9a like protein 1 antibody
      • G9a like protein antibody
      • G9a-like protein 1 antibody
      • GLP 1 antibody
      • GLP antibody
      • GLP1 antibody
      • H3 K9 HMTase 5 antibody
      • H3-K9-HMTase 5 antibody
      • Histone H3 K9 methyltransferase 5 antibody
      • Histone H3-K9 methyltransferase 5 antibody
      • Histone lysine N methyltransferase H3 lysine 9 specific 5 antibody
      • Histone-lysine N-methyltransferase EHMT1 antibody
      • KIAA1876 antibody
      • Lysine N methyltransferase 1D antibody
      • Lysine N-methyltransferase 1D antibody
      • RP11 188C12.1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (ab41969)
      Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (ab41969)
      Ab41969 at 5 µg/ml staining WT mouse ES cell (Positive)
    • Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (ab41969)
      Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (ab41969)
      Ab41969 at 5µg/ml staining GLP KO mouse ES cell (Negative)

    References

    This product has been referenced in:
    • Li H  et al. Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. Nat Commun 9:1420 (2018). Read more (PubMed: 29650963) »
    • Yang Y  et al. Euchromatic histone lysine methyltransferase 1 regulates cancer development in human gastric cancer by regulating E-cadherin. Oncol Lett 15:9480-9486 (2018). Read more (PubMed: 29844834) »

    See all 13 Publications for this product

    Publishing research using ab41969? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    9 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EHMT1/GLP antibody [B0422]

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Dermal Microvascular Endothelial Cells)
    Permeabilization
    Yes - 0.1% Triton-X100
    Specification
    Human Dermal Microvascular Endothelial Cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jul 13 2017

    ChIP abreview for Anti-EHMT1/GLP antibody [B0422]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (B cells)
    Negative control
    IgG
    Specification
    B cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 5 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    Gene Promoter
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 27 2017

    Immunoprecipitation abreview for Anti-EHMT1/GLP antibody [B0422]

     Excellent
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - nuclear (Dermal Endothelial Cells (Primary))
    Total protein in input
    300 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    Dermal Endothelial Cells (Primary)
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2017

    Western blot abreview for Anti-EHMT1/GLP antibody [B0422]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Dermal Endothelial cells (Primary))
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Dermal Endothelial cells (Primary)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 21 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EHMT1/GLP antibody [B0422]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (C2C12 myoblast cells)
    Permeabilization
    No
    Specification
    C2C12 myoblast cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 07 2013

    Ok I understand fully now. Could you send the ab63161. I will then order in the second and pay for it. Do you need a PO number for this before you send the testing code for both? I will order it now, at try to get the PO by this afternoon Wales time.

    Thank you so much for your reply.
    Below are the testing discount codes for both GLP antibodies. At the bottom of the email is the information about the free of charge replacement for ab41969. A PO number is not needed on my end for sending you the...

    Read More

    Thank you for your rapid response. I appreciate the offer to send ab1220, this would be very helpful.

    I would also like to receive both untested samples. I'm not sure I completely understand what you write below. But I think you suggest to s...

    Read More

    Thank you for your reply.
    Yes, I think you are understanding this correctly.
    I will be able to replace ab41969 free of charge with one vial of either ab63161 or ab84751. I am not able to send you both. Unfortunately, we do not offer sample si...

    Read More

    Thanks for the rapid response. First I didn't find any problem with the ab1220 only ab41969. I mention it only because I've used it to run gels on all of the same samples (more samples than necessary) as a control and to show (indirectly) GLP/G9a-methy...

    Read More

    Thank you for your reply and the additional information which will be very helpful to us to evaluate the antibody.
    I have checked, but unfortunately we do not have another new lot for ab41969 in stock right now. Instead, I can send you a replaceme...

    Read More

    I've been working withAnti-KMT1D / GLP / Eu HMTase1 antibody [B0422] - ChIP Grade (ab41969) in embryonic mouse tissue. After considerable time and several attempts I've still not found the expected bands. This is despite finding very prominent bands fo...

    Read More

    Thank you for contacting us.
    I am very sorry to hear that the antibody ab41969 is giving you problems.
    Could you please let me know the following details for this antibody:
    1) How were your samples prepared (e.g. lysis buffer)?
    2) H...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up