• Product name

  • Description

    Rabbit Polyclonal to EHMT2/G9A
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide within Human EHMT2/ G9A aa 21-118. The exact sequence is proprietary.
    Database link: Q96KQ7

  • Positive control

    • 293T, A431, HeLa, HepG2 and A375 cell lysates. HeLa cells. HeLa xenograft tissue.



Our Abpromise guarantee covers the use of ab183889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 132 kDa.


  • Function

    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
  • Tissue specificity

    Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 7 ANK repeats.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain

    The SET domain mediates interaction with WIZ.
    The ANK repeats bind H3K9me1 and H3K9me2.
  • Post-translational

    Methylated at Lys-185; automethylated.
  • Cellular localization

    Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ankyrin repeat containing protein antibody
    • Bat 8 antibody
    • Bat8 antibody
    • C6orf30 antibody
    • DKFZp686H08213 antibody
    • EHMT 2 antibody
    • Ehmt2 antibody
    • EHMT2_HUMAN antibody
    • Euchromatic histone lysine methyltransferase 2 antibody
    • Euchromatic histone lysine N methyltransferase 2 antibody
    • Euchromatic histone-lysine N-methyltransferase 2 antibody
    • FLJ35547 antibody
    • G 9a antibody
    • G9 a antibody
    • G9a protein antibody
    • G9A antibody
    • G9A histone methyltransferase antibody
    • GAT8 antibody
    • H3 K9 HMTase 3 antibody
    • H3-K9-HMTase 3 antibody
    • Histone H3 K9 methyltransferase 3 antibody
    • Histone H3 K9 methyltransferase3 antibody
    • Histone H3-K9 methyltransferase 3 antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase EHMT2 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody
    • Histone-lysine N-methyltransferase EHMT2 antibody
    • HLA B associated transcript 8 antibody
    • HLA-B-associated transcript 8 antibody
    • KMT 1C antibody
    • KMT1 C antibody
    • Lysine N methyltransferase 1C antibody
    • Lysine N-methyltransferase 1C antibody
    • NG 36 antibody
    • NG36 antibody
    • Protein G9a antibody
    see all


  • All lanes : Anti-EHMT2/G9A antibody (ab183889) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate/extract
    Lane 2 : A431 whole cell lysate/extract
    Lane 3 : HeLa whole cell lysate/extract
    Lane 4 : HepG2 whole cell lysate/extract
    Lane 5 : A375 whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 132 kDa

  • Western blot analysis on immunoprecipitation. IP sample: 2 mg of HeLa whole cell lysate/extract. Lanes: 1) 40 μg of HeLa whole cell lysate/extract. 2) control with 2 μg of pre-immune rabbit IgG. 3) immunoprecipitation of KMT1C / G9a with 2 μg of ab183889. KMT1C / G9a immunoprecipitate was detected by ab183889 at a 1/1000 dilution.

  • Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde for 5 minutes, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.

  • Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution.


ab183889 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Human Cell lysate - whole cell (293T)
Total protein in input
20 µg
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Jan 08 2016

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