Key features and details
- Rabbit Polyclonal to EHMT2/G9A
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EHMT2/G9A antibody
See all EHMT2/G9A primary antibodies
DescriptionRabbit Polyclonal to EHMT2/G9A
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide within Human EHMT2/ G9A aa 21-118. The exact sequence is proprietary.
Database link: Q96KQ7
- 293T, A431, HeLa, HepG2 and A375 cell lysates. HeLa cells. HeLa xenograft tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 132 kDa.|
FunctionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Tissue specificityExpressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
DomainThe SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2.
modificationsMethylated at Lys-185; automethylated.
Cellular localizationNucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
- Information by UniProt
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All lanes : Anti-EHMT2/G9A antibody (ab183889) at 1/1000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lane 5 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 132 kDa
Western blot analysis on immunoprecipitation. IP sample: 2 mg of HeLa whole cell lysate/extract. Lanes: 1) 40 μg of HeLa whole cell lysate/extract. 2) control with 2 μg of pre-immune rabbit IgG. 3) immunoprecipitation of KMT1C / G9a with 2 μg of ab183889. KMT1C / G9a immunoprecipitate was detected by ab183889 at a 1/1000 dilution.
Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde for 5 minutes, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution.
ab183889 has not yet been referenced specifically in any publications.