Overview

  • Product name

  • Description

    Rabbit Polyclonal to EHMT2/G9A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide within Human EHMT2/ G9A aa 21-118. The exact sequence is proprietary.
    Database link: Q96KQ7

  • Positive control

    • 293T, A431, HeLa, HepG2 and A375 cell lysates. HeLa cells. HeLa xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab183889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 132 kDa.

Target

  • Function

    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
  • Tissue specificity

    Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 7 ANK repeats.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain

    The SET domain mediates interaction with WIZ.
    The ANK repeats bind H3K9me1 and H3K9me2.
  • Post-translational
    modifications

    Methylated at Lys-185; automethylated.
  • Cellular localization

    Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ankyrin repeat containing protein antibody
    • Bat 8 antibody
    • Bat8 antibody
    • C6orf30 antibody
    • DKFZp686H08213 antibody
    • EHMT 2 antibody
    • Ehmt2 antibody
    • EHMT2_HUMAN antibody
    • Euchromatic histone lysine methyltransferase 2 antibody
    • Euchromatic histone lysine N methyltransferase 2 antibody
    • Euchromatic histone-lysine N-methyltransferase 2 antibody
    • FLJ35547 antibody
    • G 9a antibody
    • G9 a antibody
    • G9a protein antibody
    • G9A antibody
    • G9A histone methyltransferase antibody
    • GAT8 antibody
    • H3 K9 HMTase 3 antibody
    • H3-K9-HMTase 3 antibody
    • Histone H3 K9 methyltransferase 3 antibody
    • Histone H3 K9 methyltransferase3 antibody
    • Histone H3-K9 methyltransferase 3 antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase EHMT2 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody
    • Histone-lysine N-methyltransferase EHMT2 antibody
    • HLA B associated transcript 8 antibody
    • HLA-B-associated transcript 8 antibody
    • KMT 1C antibody
    • KMT1 C antibody
    • Lysine N methyltransferase 1C antibody
    • Lysine N-methyltransferase 1C antibody
    • NG 36 antibody
    • NG36 antibody
    • Protein G9a antibody
    see all

Images

  • All lanes : Anti-EHMT2/G9A antibody (ab183889) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate/extract
    Lane 2 : A431 whole cell lysate/extract
    Lane 3 : HeLa whole cell lysate/extract
    Lane 4 : HepG2 whole cell lysate/extract
    Lane 5 : A375 whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 132 kDa

  • Western blot analysis on immunoprecipitation. IP sample: 2 mg of HeLa whole cell lysate/extract. Lanes: 1) 40 μg of HeLa whole cell lysate/extract. 2) control with 2 μg of pre-immune rabbit IgG. 3) immunoprecipitation of KMT1C / G9a with 2 μg of ab183889. KMT1C / G9a immunoprecipitate was detected by ab183889 at a 1/1000 dilution.

  • Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde for 5 minutes, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.

  • Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution.

References

ab183889 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
20 µg
Immuno-precipitation step
Protein A
Specification
293T

Abcam user community

Verified customer

Submitted Jan 08 2016

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