Anti-EHMT2/G9A antibody (ab183889)
Key features and details
- Rabbit polyclonal to EHMT2/G9A
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EHMT2/G9A antibody
See all EHMT2/G9A primary antibodies -
Description
Rabbit polyclonal to EHMT2/G9A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Synthetic peptide within Human EHMT2/ G9A aa 21-118. The exact sequence is proprietary.
Database link: Q96KQ7 -
Positive control
- 293T, A431, HeLa, HepG2 and A375 cell lysates. HeLa cells. HeLa xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IP | (1) |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 132 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 132 kDa. |
Target
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Function
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. -
Tissue specificity
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Domain
The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2. -
Post-translational
modificationsMethylated at Lys-185; automethylated. -
Cellular localization
Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 514062 Cow
- Entrez Gene: 10919 Human
- Entrez Gene: 110147 Mouse
- Entrez Gene: 361798 Rat
- Omim: 604599 Human
- SwissProt: Q96KQ7 Human
- SwissProt: Q9Z148 Mouse
- Unigene: 709218 Human
see all -
Alternative names
- Ankyrin repeat containing protein antibody
- Bat 8 antibody
- Bat8 antibody
see all
Images
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All lanes : Anti-EHMT2/G9A antibody (ab183889) at 1/1000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lane 5 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 132 kDa -
Western blot analysis on immunoprecipitation. IP sample: 2 mg of HeLa whole cell lysate/extract. Lanes: 1) 40 μg of HeLa whole cell lysate/extract. 2) control with 2 μg of pre-immune rabbit IgG. 3) immunoprecipitation of KMT1C / G9a with 2 μg of ab183889. KMT1C / G9a immunoprecipitate was detected by ab183889 at a 1/1000 dilution.
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Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde for 5 minutes, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183889 has not yet been referenced specifically in any publications.