Overview

  • Product name

  • Description

    Rabbit polyclonal to EHMT2/G9A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ChIP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment within Human EHMT2/G9A (internal sequence). The exact sequence is proprietary.
    Database link: Q96KQ7

  • Positive control

    • WB: A431, HeLa, HepG2 and HEK-293T whole cell lysates; HEK-293T, Neuro-2a, C8D30, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. IP: HeLa whole cell lysate (ab150035). IHC-P: Human lung carcinoma tissue. ICC/IF: HeLa cells. ChIP: HeLa chromatin extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 132 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ChIP Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
  • Tissue specificity

    Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 7 ANK repeats.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain

    The SET domain mediates interaction with WIZ.
    The ANK repeats bind H3K9me1 and H3K9me2.
  • Post-translational
    modifications

    Methylated at Lys-185; automethylated.
  • Cellular localization

    Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ankyrin repeat containing protein antibody
    • Bat 8 antibody
    • Bat8 antibody
    • C6orf30 antibody
    • DKFZp686H08213 antibody
    • EHMT 2 antibody
    • Ehmt2 antibody
    • EHMT2_HUMAN antibody
    • Euchromatic histone lysine methyltransferase 2 antibody
    • Euchromatic histone lysine N methyltransferase 2 antibody
    • Euchromatic histone-lysine N-methyltransferase 2 antibody
    • FLJ35547 antibody
    • G 9a antibody
    • G9 a antibody
    • G9a protein antibody
    • G9A antibody
    • G9A histone methyltransferase antibody
    • GAT8 antibody
    • H3 K9 HMTase 3 antibody
    • H3-K9-HMTase 3 antibody
    • Histone H3 K9 methyltransferase 3 antibody
    • Histone H3 K9 methyltransferase3 antibody
    • Histone H3-K9 methyltransferase 3 antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase EHMT2 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody
    • Histone-lysine N-methyltransferase EHMT2 antibody
    • HLA B associated transcript 8 antibody
    • HLA-B-associated transcript 8 antibody
    • KMT 1C antibody
    • KMT1 C antibody
    • Lysine N methyltransferase 1C antibody
    • Lysine N-methyltransferase 1C antibody
    • NG 36 antibody
    • NG36 antibody
    • Protein G9a antibody
    see all

Images

  • All lanes : Anti-EHMT2/G9A antibody (ab229455) at 1/2000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lanes 2-3 : EHMT2/G9A shRNA-transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 132 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-EHMT2/G9A antibody (ab229455) at 1/3000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 132 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-EHMT2/G9A antibody (ab229455) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 132 kDa



    5% SDS-PAGE

  • Paraffin-embedded human lung carcinoma tissue stained for EHMT2/G9A with ab229455 at 1/250 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EHMT2/G9A (green) using ab229455 at 1/1000 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue). The cytoskeleton is stained with phalloidin at 1/200 dilution (red).

  • EHMT2/G9A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 3 μg ab229455. Western blot was performed from the immunoprecipitate using ab229455 at 1/1000 dilution. Anti-rabbit IgG (HRP)  was used as a secondary reagent.

    Lane 1: Control IP in HeLa whole cell lysate with 3 μg of pre-immune rabbit IgG.

    Lane 2: ab229455 IP in HeLa whole cell lysate.

    5% SDS-PAGE gel.

  • ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or ab229455. The precipitated DNA was detected by PCR with primer set targeting to FXN.

References

ab229455 has not yet been referenced specifically in any publications.

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