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Fusion protein corresponding to Human EHMT2/ G9A aa 831-1001.
Database link: Q96KQ7
Our Abpromise guarantee covers the use of ab40542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|IHC-P||1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/2000 - 1/2500. Predicted molecular weight: 132 kDa. For endogeneous detection (without G9a over expression): use nuclear extracts and block overnight or perform IP prior to WB.|
ab40542 (1:2000) staining EHMT2/ G9A in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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