Anti-EHMT2/G9A antibody (ab40542)
Key features and details
- Rabbit polyclonal to EHMT2/G9A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHMT2/G9A antibody
See all EHMT2/G9A primary antibodies -
Description
Rabbit polyclonal to EHMT2/G9A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human EHMT2/ G9A aa 831-1001.
Database link: Q96KQ7 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (5) |
1/2000 - 1/2500. Predicted molecular weight: 132 kDa. For endogeneous detection (without G9a over expression): use nuclear extracts and block overnight or perform IP prior to WB.
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Notes |
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IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/2000 - 1/2500. Predicted molecular weight: 132 kDa. For endogeneous detection (without G9a over expression): use nuclear extracts and block overnight or perform IP prior to WB. |
Target
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Function
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. -
Tissue specificity
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Domain
The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2. -
Post-translational
modificationsMethylated at Lys-185; automethylated. -
Cellular localization
Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 10919 Human
- Omim: 604599 Human
- SwissProt: Q96KQ7 Human
- Unigene: 709218 Human
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Alternative names
- Ankyrin repeat containing protein antibody
- Bat 8 antibody
- Bat8 antibody
see all
Images
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All lanes : Anti-EHMT2/G9A antibody (ab40542) at 1/2500 dilution
Lane 1 : Untransfected HeLa cell extract
Lane 2 : G9a transfected HeLa cell extract
Predicted band size: 132 kDa
Observed band size: 132 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
ab40542 (1:2000) staining EHMT2/ G9A in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab40542 has been referenced in 45 publications.
- Anderson EM et al. The histone methyltransferase G9a mediates stress-regulated alcohol drinking. Addict Biol 27:e13060 (2022). PubMed: 34013595
- Yang F et al. The gut microbiota mediates protective immunity against tuberculosis via modulation of lncRNA. Gut Microbes 14:2029997 (2022). PubMed: 35343370
- Yang N et al. An interplay between BRD4 and G9a regulates skeletal myogenesis. Front Cell Dev Biol 10:978931 (2022). PubMed: 36158208
- Pu FF et al. SP1-induced long non-coding RNA SNHG6 facilitates the carcinogenesis of chondrosarcoma through inhibiting KLF6 by recruiting EZH2. Cell Death Dis 12:59 (2021). PubMed: 33431838
- Gimeno-Valiente F et al. Epigenetic Mechanisms Are Involved in the Oncogenic Properties of ZNF518B in Colorectal Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 33801071