Product nameAnti-EHMT2/G9A antibody [EPR4019(2)]
See all EHMT2/G9A primary antibodies
DescriptionRabbit monoclonal [EPR4019(2)] to EHMT2/G9A
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide within Human EHMT2/G9A aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q96KQ7
- HeLa, NCCIT, HepG2, and 293T cell lysates. Human gastric adenocarcinoma tissue and Human lung squamous carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 132 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Tissue specificityExpressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
DomainThe SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2.
modificationsMethylated at Lys-185; automethylated.
Cellular localizationNucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
- Information by UniProt
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EHMT2/G9A knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133482 observed at 170 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133482 was shown to recognize EHMT2/G9A when EHMT2/G9A knockout samples were used, along with additional cross-reactive bands. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab133482 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NCCIT cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat-anti-rabbit at 1/2000 dilution
Predicted band size: 132 kDa
Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma tissue labelled with ab133482 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded lung squamous carcinoma tissue labelled with ab133482 at 1/50 dilution.
This product has been referenced in:
- Qin J et al. Clinicopathological significance of G9A expression in colorectal carcinoma. Oncol Lett 15:8611-8619 (2018). Read more (PubMed: 29805595) »
- Karthik IP et al. E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases. J Biol Chem 293:5624-5635 (2018). Read more (PubMed: 29467226) »