Overview

  • Product name

    Anti-EHMT2/G9A antibody [EPR4019(2)]
    See all EHMT2/G9A primary antibodies
  • Description

    Rabbit monoclonal [EPR4019(2)] to EHMT2/G9A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EHMT2/G9A aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q96KQ7

  • Positive control

    • HeLa, NCCIT, HepG2, and 293T cell lysates. Human gastric adenocarcinoma tissue and Human lung squamous carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 132 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
    • Tissue specificity

      Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
    • Sequence similarities

      Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
      Contains 7 ANK repeats.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
    • Domain

      The SET domain mediates interaction with WIZ.
      The ANK repeats bind H3K9me1 and H3K9me2.
    • Post-translational
      modifications

      Methylated at Lys-185; automethylated.
    • Cellular localization

      Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ankyrin repeat containing protein antibody
      • Bat 8 antibody
      • Bat8 antibody
      • C6orf30 antibody
      • DKFZp686H08213 antibody
      • EHMT 2 antibody
      • Ehmt2 antibody
      • EHMT2_HUMAN antibody
      • Euchromatic histone lysine methyltransferase 2 antibody
      • Euchromatic histone lysine N methyltransferase 2 antibody
      • Euchromatic histone-lysine N-methyltransferase 2 antibody
      • FLJ35547 antibody
      • G 9a antibody
      • G9 a antibody
      • G9a protein antibody
      • G9A antibody
      • G9A histone methyltransferase antibody
      • GAT8 antibody
      • H3 K9 HMTase 3 antibody
      • H3-K9-HMTase 3 antibody
      • Histone H3 K9 methyltransferase 3 antibody
      • Histone H3 K9 methyltransferase3 antibody
      • Histone H3-K9 methyltransferase 3 antibody
      • Histone lysine N methyltransferase antibody
      • Histone lysine N methyltransferase EHMT2 antibody
      • Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody
      • Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody
      • Histone-lysine N-methyltransferase EHMT2 antibody
      • HLA B associated transcript 8 antibody
      • HLA-B-associated transcript 8 antibody
      • KMT 1C antibody
      • KMT1 C antibody
      • Lysine N methyltransferase 1C antibody
      • Lysine N-methyltransferase 1C antibody
      • NG 36 antibody
      • NG36 antibody
      • Protein G9a antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: EHMT2/G9A knockout HAP1 cell lysate (20 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133482 observed at 170 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab133482 was shown to recognize EHMT2/G9A when EHMT2/G9A knockout samples were used, along with additional cross-reactive bands. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab133482 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : NCCIT cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat-anti-rabbit at 1/2000 dilution

      Predicted band size: 132 kDa

    • Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma tissue labelled with ab133482 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded lung squamous carcinoma tissue labelled with ab133482 at 1/50 dilution.

    References

    This product has been referenced in:

    • Qin J  et al. Clinicopathological significance of G9A expression in colorectal carcinoma. Oncol Lett 15:8611-8619 (2018). Read more (PubMed: 29805595) »
    • Karthik IP  et al. E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases. J Biol Chem 293:5624-5635 (2018). Read more (PubMed: 29467226) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    15 µg
    Treatment
    siLuc and siG9a
    Specification
    U2OS cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Mar 23 2015

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