Recombinant Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
![Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-389662-anti-ehmt2g9a-antibody-epr40192-western-blot-human-lysate.jpg "Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4019(2)] to EHMT2/G9A - ChIP Grade
- Suitable for: WB, IHC-P, ChIP-sequencing
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EHMT2/G9A antibody [EPR4019(2)]
See all EHMT2/G9A primary antibodies -
Description
Rabbit monoclonal [EPR4019(2)] to EHMT2/G9A - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ChIP-sequencingmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EHMT2/G9A aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q96KQ7 -
Positive control
- WB: HeLa, NCCIT, HepG2, and 293T cell lysates. IHC-P: Human gastric adenocarcinoma and lung squamous tissue. ChIP-seq: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4019(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 132 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ChIP-sequencing |
Use 8µg for 107 cells.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 132 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ChIP-sequencing
Use 8µg for 107 cells. |
Target
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Function
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. -
Tissue specificity
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Domain
The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2. -
Post-translational
modificationsMethylated at Lys-185; automethylated. -
Cellular localization
Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 10919 Human
- Omim: 604599 Human
- SwissProt: Q96KQ7 Human
- Unigene: 709218 Human
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Alternative names
- Ankyrin repeat containing protein antibody
- Bat 8 antibody
- Bat8 antibody
see all
Images
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Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EHMT2/G9A knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 132 kDaLanes 1-4: Merged signal (red and green). Green - ab133482 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133482 Anti-EHMT2/G9A antibody [EPR4019(2)] was shown to specifically react with EHMT2/G9A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265149 (knockout cell lysate ab257080) was used. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab133482 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![ChIP-sequencing - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-482560-antiehmt2g9aantibodyepr40192chipgradechipseqhumanhela.jpg)
ChIP-sequencing - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 8 µg of ab133482 [EPR4019(2)]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-147129-anti-ehmt2-g9a-antibody-epr40192-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)Immunohistochemical analysis of paraffin-embedded lung squamous carcinoma tissue labelled with ab133482 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-259087-anti-ehmt2-g9a-antibody-epr40192-western-blot.jpg)
Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : EHMT2/G9A knockout HAP1 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 132 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133482 observed at 170 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133482 was shown to recognize EHMT2/G9A when EHMT2/G9A knockout samples were used, along with additional cross-reactive bands. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab133482 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-147131-anti-ehmt2-g9a-antibody-epr40192-western-blot.jpg)
Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NCCIT cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat-anti-rabbit at 1/2000 dilution
Predicted band size: 132 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-147130-anti-ehmt2-g9a-antibody-epr40192-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma tissue labelled with ab133482 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)](https://www.abcam.com/ps/products/133/ab133482/Images/ab133482-6-benefits-of-recombinant-antibodies.png)
Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab133482 has been referenced in 9 publications.
- Xu P et al. Prognostic value of lymphocyte-to-monocyte ratio and histone methyltransferase G9a histone methyltransferase in patients with double expression lymphoma: A retrospective observational study. Medicine (Baltimore) 100:e24449 (2021). PubMed: 33530253
- Xia S et al. SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells. Cell Death Dis 12:570 (2021). PubMed: 34108444
- Bellamy J et al. Increased Efficacy of Histone Methyltransferase G9a Inhibitors Against MYCN-Amplified Neuroblastoma. Front Oncol 10:818 (2020). PubMed: 32537432
- Qin J et al. Clinicopathological significance of G9A expression in colorectal carcinoma. Oncol Lett 15:8611-8619 (2018). PubMed: 29805595
- Karthik IP et al. E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases. J Biol Chem 293:5624-5635 (2018). PubMed: 29467226
- Coward WR et al. Interplay between EZH2 and G9a Regulates CXCL10 Gene Repression in Idiopathic Pulmonary Fibrosis. Am J Respir Cell Mol Biol 58:449-460 (2018). PubMed: 29053336
- Sakamaki JI et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell 66:517-532.e9 (2017). PubMed: 28525743
- Bai K et al. Association of Histone Methyltransferase G9a and Overall Survival After Liver Resection of Patients With Hepatocellular Carcinoma With a Median Observation of 40 Months. Medicine (Baltimore) 95:e2493 (2016). IHC . PubMed: 26765460
- Artal-Martinez de Narvajas A et al. Epigenetic regulation of autophagy by the methyltransferase G9a. Mol Cell Biol 33:3983-93 (2013). WB ; Human . PubMed: 23918802
