Anti-EI2BL/MRI1 antibody (ab187894)
Key features and details
- Rabbit polyclonal to EI2BL/MRI1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EI2BL/MRI1 antibody -
Description
Rabbit polyclonal to EI2BL/MRI1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Cynomolgus monkey -
Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human EI2BL/MRI1 aa 250 to the C-terminus.
Database link: Q9BV20 -
Positive control
- Human thyroid gland tissue; RT-4, U-251MG sp cell lysates; U-2 OS cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Independently from catalytic activity, promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation and stress fiber turnover. -
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6. -
Sequence similarities
Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily. -
Cellular localization
Nucleus. Cytoplasm. Cell projection. Primarily nuclear, but cytoplasmic in cancer cells, with enrichment at leading edge of the plasma membrane in late stage tumor cells. - Information by UniProt
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Database links
- Entrez Gene: 534734 Cow
- Entrez Gene: 84245 Human
- Entrez Gene: 288912 Rat
- Omim: 615105 Human
- SwissProt: Q2NL31 Cow
- SwissProt: Q9BV20 Human
- SwissProt: Q5HZE4 Rat
- Unigene: 439370 Human
see all -
Alternative names
- EI2BL antibody
- M1Pi antibody
- Mediator of RhoA-dependent invasion antibody
see all
Images
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All lanes : Anti-EI2BL/MRI1 antibody (ab187894) at 1/1000 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251MG sp cell lysate -
Immunofluorescent analysis of Human cell line U-2 OS labeling EI2BL/MRI1 with ab187894 at 4µg/ml.
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human thyroid gland tssue labeling EI2BL/MRI1 with ab187894 at 1/1000 dilution.
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Immunofluorescent analysis of Human cell line U-2 OS labeling EI2BL/MRI1 with ab187894 at 4µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab187894 has been referenced in 1 publication.
- Li HR et al. Overexpression of EI2BL promoted human non-small cell lung cancer progression by inducing cell EMT phenotype. J Clin Pathol 73:139-146 (2020). PubMed: 31586937