Overview

  • Product name
  • Description
    Rabbit polyclonal to EID1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    GSGSRELSLRPSRSGA

    conjugated to KLH, corresponding to C terminal amino acids 34-49 of Human EID1.

  • Positive control
    • EID1 transfected Cos1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab14454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 22 kDa.

Target

  • Function
    Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
  • Tissue specificity
    Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.
  • Developmental stage
    Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).
  • Post-translational
    modifications
    Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
  • Cellular localization
    Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 21 kDa pRb associated protein antibody
    • 21 kDa pRb-associated protein antibody
    • C15orf3 antibody
    • CBP antibody
    • Chromosome 15 open reading frame 3 antibody
    • CREBBP/EP300 inhibitor 1 antibody
    • CREBBP/EP300 inhibitory protein 1 antibody
    • CRI 1 antibody
    • CRI1 antibody
    • E1A like inhibitor of differentiation antibody
    • E1A-like inhibitor of differentiation 1 antibody
    • EID 1 antibody
    • EID-1 antibody
    • EID1 antibody
    • EID1_HUMAN antibody
    • EP300 interacting inhibitor of differentiation 1 antibody
    • EP300-interacting inhibitor of differentiation 1 antibody
    • IRO45620 antibody
    • MGC138883 antibody
    • MGC138884 antibody
    • NB4 apoptosis related protein antibody
    • PNAS 22 antibody
    • PNAS22 antibody
    • PTD014 antibody
    • Rb and p300 binding protein EID 1 antibody
    • Rb and p300 binding protein EID1 antibody
    • RBP21 antibody
    • Retinoblastoma protein associated protein antibody
    see all

References

ab14454 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

BATCH NUMBER Lot79677 DESCRIPTION OF THE PROBLEM No signal SAMPLE We tested its reactivity towards the endogenous EID1 in 100microgram lysates from HeLa, U937 and 293 cells, and also on 293 cells over-expressing high levels of exogenous EID1. PRIMARY ANTIBODY 4 microgram/ ml, 1h or overnight DETECTION METHOD ECL-Pierce's super-sensitive ECL reagent. POSITIVE AND NEGATIVE CONTROLS USED antibody to this protein from an alternate source worked just fine under same conditions. ANTIBODY STORAGE CONDITIONS 4c SAMPLE PREPARATION RIPA +protease inhibitors, boiling 5 min with protein sample buffer that contains beta mercaptoethanol AMOUNT OF PROTEIN LOADED 100 microgram total protein ELECTROPHORESIS/GEL CONDITIONS 10%, 12% SDS PAGE Gel TRANSFER AND BLOCKING CONDITIONS 1.5 h transfer Tris glycin buffer, blocking with 10% milk SECONDARY ANTIBODY We used horseradish peroxidase linked-protein A (Amersham) as our secondary. Pierce's super-sensitive ECL reagent. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES We used this product for western blots, according to your recommendations in the product datasheet, at a concentration of 4microgram/ml (i.e. the higher concentration). We tested its reactivity towards the endogenous EID1 in 100microgram lysates from HeLa, U937 and 293 cells, and also on 293 cells over-expressing high levels of exogenous EID1. We used horseradish peroxidase linked-protein A (Amersham) as our secondary, and Pierce's super-sensitive ECL reagent. However, we failed to detect any signal with your antibody, although a second antibody to this protein from an alternate source worked just fine. As this product does not seem to work, I would like to be able to return it and get a refund. Thank you for yor assistance,

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Answer

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire and for providing details of your Western blot protocol. I am very sorry to hear that you are having problem with this antibody. I have looked through your procedure and it looks absolutely fine. I have also checked FedEx website and the delivery details in our computer system and found the following. The package was held up at the customs for clearance and checking and it may well be that during this time the antibody was not stored properly. Since you have an alternative antibody which works nicely, certainly I can offer you reimbursement in this case. I can offer you either a credit note which you can use for your next purchase or a full refund. Please do let me know how you would prefer to proceed and also confirm your Purchase Order Number and/or your Abcam Order Number. I am looking forward to hearing from you soon.

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Question
Answer

Thank you for your enquiry regarding ab14454. We have not yet tested the cross-reactivity for this antibody with mouse tissue but expect the antibody to work as there is 90% similarity between mouse and human (which we have tested). Should you decide to test an antibody in mouse please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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