Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Catfish, Rainbow trout, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human eIF-6 aa 150-200.

  • Positive control

    • HeLa or NIH3T3 whole cell lysate
  • General notes

    Previously labelled as Integrin beta 4 binding protein. 

Properties

Applications

Our Abpromise guarantee covers the use of ab109593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
  • Tissue specificity

    Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
  • Sequence similarities

    Belongs to the eIF-6 family.
  • Post-translational
    modifications

    Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • b(2)gcn antibody
    • B(2)GCN homolog antibody
    • B4 integrin interactor antibody
    • Binding protein of beta-4 integrin antibody
    • CAB antibody
    • eIF-6 antibody
    • EIF3A antibody
    • EIF6 antibody
    • Eukaryotic translation initiation factor 3A antibody
    • Eukaryotic translation initiation factor 6 antibody
    • IF6_HUMAN antibody
    • Integrin beta 4 binding protein antibody
    • ITGB4BP antibody
    • OK/SW-cl.27 antibody
    • p27 beta 4 integrin binding protein antibody
    • p27(BBP) antibody
    • p27BBP antibody
    • RP4-614O4.1 antibody
    see all

Images

  • All lanes : Anti-eIF-6 antibody (ab109593) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 27 kDa


    Exposure time: 3 minutes
  • Detection of eIF-6 by Western Blot of Immunprecipitate.
    Lane 1: ab109593 at 1µg/ml staining eIF-6 in HeLa whole cell lysate immunoprecipitated using ab109593 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab109593 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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