Key features and details
- Rabbit polyclonal to eIF-6
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF-6 antibody
See all eIF-6 primary antibodies
DescriptionRabbit polyclonal to eIF-6
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human eIF-6 aa 50-100. The exact sequence is proprietary. NP_852133.1
Database link: P56537
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245532 was affinity purified using an epitope specific to eIF-6 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 27 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
Tissue specificityExpressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Sequence similaritiesBelongs to the eIF-6 family.
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export.
Cellular localizationCytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
- Information by UniProt
- b(2)gcn antibody
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All lanes : Anti-eIF-6 antibody (ab245532) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 3 minutes
eIF-6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245532 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245532 at 1 µg/ml.
Lane 1: ab245532 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for eIF-6 using ab245532 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: Hematoxylin (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245532 has not yet been referenced specifically in any publications.