Anti-eIF-6 antibody (ab245532)

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Overview

  • Product name

    Anti-eIF-6 antibody
    See all eIF-6 primary antibodies

  • Description

    Rabbit polyclonal to eIF-6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human eIF-6 aa 50-100. The exact sequence is proprietary. NP_852133.1
    Database link: P56537

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.

  • Tissue specificity

    Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

  • Sequence similarities

    Belongs to the eIF-6 family.

  • Post-translational
    modifications

    Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.

  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
  • Information by UniProt

  • Database links

    • Alternative names

      • b(2)gcn antibody
      • B(2)GCN homolog antibody
      • B4 integrin interactor antibody
      • Binding protein of beta-4 integrin antibody
      • CAB antibody
      • eIF-6 antibody
      • EIF3A antibody
      • EIF6 antibody
      • Eukaryotic translation initiation factor 3A antibody
      • Eukaryotic translation initiation factor 6 antibody
      • IF6_HUMAN antibody
      • Integrin beta 4 binding protein antibody
      • ITGB4BP antibody
      • OK/SW-cl.27 antibody
      • p27 beta 4 integrin binding protein antibody
      • p27(BBP) antibody
      • p27BBP antibody
      • RP4-614O4.1 antibody
      see all

    Images

    • Western blot - Anti-eIF-6 antibody (ab245532)
      Western blot - Anti-eIF-6 antibody (ab245532)
      All lanes : Anti-eIF-6 antibody (ab245532) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 27 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-eIF-6 antibody (ab245532)
      Immunoprecipitation - Anti-eIF-6 antibody (ab245532)

      eIF-6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245532 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245532 at 1 µg/ml.

      Lane 1: ab245532 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF-6 antibody (ab245532)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF-6 antibody (ab245532)

      Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for eIF-6 using ab245532 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: Hematoxylin (blue).

    References

    ab245532 has not yet been referenced specifically in any publications.

    Publishing research using ab245532? Please let us know so that we can cite the reference in this datasheet.

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