Anti-eIF-6 antibody (ab77298)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-eIF-6 antibody
See all eIF-6 primary antibodies -
DescriptionRabbit polyclonal to eIF-6
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow
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Immunogen
Synthetic peptide corresponding to Human eIF-6 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90213) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: SW480; Caco2; HeLa; HEK293; HepG2; LOVO; K562.
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General notes
Previously labelled as Integrin beta 4 binding protein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab77298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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FunctionBinds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
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Tissue specificityExpressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
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Sequence similaritiesBelongs to the eIF-6 family.
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Post-translational
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export. -
Cellular localizationCytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
- Information by UniProt
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Database links
- Entrez Gene: 286811 Cow
- Entrez Gene: 3692 Human
- Entrez Gene: 16418 Mouse
- Entrez Gene: 305506 Rat
- Entrez Gene: 100302338 Sheep
- Omim: 602912 Human
- SwissProt: Q9TU47 Cow
- SwissProt: P56537 Human
see all -
Alternative names
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
see all
Images
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Western blot - Anti-eIF-6 antibody (ab77298)All lanes : Anti-eIF-6 antibody (ab77298) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)ICC/IF image of ab77298 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.
Datasheets and documents
References
This product has been referenced in:
- Tarallo R et al. The nuclear receptor ERß engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol 18:189 (2017). WB ; Human . Read more (PubMed: 29017520) »
