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    eif-6-antibody-ab77298.pdf

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Neuroscience Cell Adhesion Proteins Receptors Integrins Beta Integrins
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Anti-eIF-6 antibody (ab77298)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-eIF-6 antibody (ab77298)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)

Key features and details

  • Rabbit polyclonal to eIF-6
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-eIF-6 antibody
    See all eIF-6 primary antibodies
  • Description

    Rabbit polyclonal to eIF-6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human eIF-6 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90213)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: SW480; Caco2; HeLa; HEK293; HepG2; LOVO; K562.
  • General notes

    Previously labelled as Integrin beta 4 binding protein. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Receptors
    • Integrins
    • Beta Integrins
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab77298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Sheep
Cow
Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
  • Tissue specificity

    Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
  • Sequence similarities

    Belongs to the eIF-6 family.
  • Post-translational
    modifications

    Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
  • Target information above from: UniProt accession P56537 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 286811 Cow
    • Entrez Gene: 3692 Human
    • Entrez Gene: 16418 Mouse
    • Entrez Gene: 305506 Rat
    • Entrez Gene: 100302338 Sheep
    • Omim: 602912 Human
    • SwissProt: Q9TU47 Cow
    • SwissProt: P56537 Human
    • SwissProt: O55135 Mouse
    • SwissProt: Q3KRD8 Rat
    • Unigene: 654848 Human
    • Unigene: 271674 Mouse
    • Unigene: 190833 Rat
    see all
  • Alternative names

    • b(2)gcn antibody
    • B(2)GCN homolog antibody
    • B4 integrin interactor antibody
    • Binding protein of beta-4 integrin antibody
    • CAB antibody
    • eIF-6 antibody
    • EIF3A antibody
    • EIF6 antibody
    • Eukaryotic translation initiation factor 3A antibody
    • Eukaryotic translation initiation factor 6 antibody
    • IF6_HUMAN antibody
    • Integrin beta 4 binding protein antibody
    • ITGB4BP antibody
    • OK/SW-cl.27 antibody
    • p27 beta 4 integrin binding protein antibody
    • p27(BBP) antibody
    • p27BBP antibody
    • RP4-614O4.1 antibody
    see all

Images

  • Western blot - Anti-eIF-6 antibody (ab77298)
    Western blot - Anti-eIF-6 antibody (ab77298)
    All lanes : Anti-eIF-6 antibody (ab77298) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)
    ICC/IF image of ab77298 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab77298? Please let us know so that we can cite the reference in this datasheet.

    ab77298 has been referenced in 1 publication.

    • Tarallo R  et al. The nuclear receptor ERß engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol 18:189 (2017). WB ; Human . PubMed: 29017520

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