Product nameAnti-eIF-6 antibody [EPR6512(B)]
See all eIF-6 primary antibodies
DescriptionRabbit monoclonal [EPR6512(B)] to eIF-6
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human eIF-6 aa 150-250.
Database link: P56537
- HeLa, RAW 264.7, K562, 293T, and HepG2 cell lysates and Human colonic adenocarcinoma tissue.
Previously labelled as Integrin beta 4 binding protein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesNot purified; provided in cell supernatant and storage buffer.
Our Abpromise guarantee covers the use of ab124839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 27 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
Tissue specificityExpressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Sequence similaritiesBelongs to the eIF-6 family.
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export.
Cellular localizationCytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
- Information by UniProt
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
All lanes : Anti-eIF-6 antibody [EPR6512(B)] (ab124839) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa
ab124839, at 1/100 to 1/250, staining eIF-6 in Human colonic adenocarcinoma by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab124839 has not yet been referenced specifically in any publications.