Recombinant Anti-eIF-6 antibody [EPR6513] (ab133321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6513] to eIF-6
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF-6 antibody [EPR6513]
See all eIF-6 primary antibodies -
Description
Rabbit monoclonal [EPR6513] to eIF-6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human eIF-6 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, 293T, HepG2, and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6513 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. -
Tissue specificity
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). -
Sequence similarities
Belongs to the eIF-6 family. -
Post-translational
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 3692 Human
- Entrez Gene: 16418 Mouse
- Entrez Gene: 305506 Rat
- Omim: 602912 Human
- SwissProt: P56537 Human
- SwissProt: O55135 Mouse
- SwissProt: Q3KRD8 Rat
- Unigene: 654848 Human
see all -
Alternative names
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
see all
Images
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All lanes : Anti-eIF-6 antibody [EPR6513] (ab133321) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133321 has not yet been referenced specifically in any publications.