Overview

  • Product name
    Anti-eIF-6 antibody [EPR6513]
    See all eIF-6 primary antibodies
  • Description
    Rabbit monoclonal [EPR6513] to eIF-6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF-6 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa, 293T, HepG2, and K562 cell lysates.
  • General notes

     

     

    Previously labelled as Integrin beta 4 binding protein. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
    • Tissue specificity
      Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
    • Sequence similarities
      Belongs to the eIF-6 family.
    • Post-translational
      modifications
      Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.
    • Cellular localization
      Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • b(2)gcn antibody
      • B(2)GCN homolog antibody
      • B4 integrin interactor antibody
      • Binding protein of beta-4 integrin antibody
      • CAB antibody
      • eIF-6 antibody
      • EIF3A antibody
      • EIF6 antibody
      • Eukaryotic translation initiation factor 3A antibody
      • Eukaryotic translation initiation factor 6 antibody
      • IF6_HUMAN antibody
      • Integrin beta 4 binding protein antibody
      • ITGB4BP antibody
      • OK/SW-cl.27 antibody
      • p27 beta 4 integrin binding protein antibody
      • p27(BBP) antibody
      • p27BBP antibody
      • RP4-614O4.1 antibody
      see all

    Images

    • All lanes : Anti-eIF-6 antibody [EPR6513] (ab133321) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 27 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133321 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133321.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up