Product nameAnti-eIF-6 antibody [EPR6513]
See all eIF-6 primary antibodies
DescriptionRabbit monoclonal [EPR6513] to eIF-6
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human eIF-6 aa 200-300 (C terminal). The exact sequence is proprietary.
- HeLa, 293T, HepG2, and K562 cell lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
|IP||1/10 - 1/100.|
FunctionBinds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
Tissue specificityExpressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Sequence similaritiesBelongs to the eIF-6 family.
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export.
Cellular localizationCytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
- Information by UniProt
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
All lanes : Anti-eIF-6 antibody [EPR6513] (ab133321) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133321 has not yet been referenced specifically in any publications.