Recombinant Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
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Overview
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Product name
Anti-eIF-6 antibody [EPR6513] - BSA and Azide free
See all eIF-6 primary antibodies -
Description
Rabbit monoclonal [EPR6513] to eIF-6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human eIF-6 aa 200-300 (C terminal). The exact sequence is proprietary.
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General notes
Ab248473 is the carrier-free version of ab133321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248473 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.70 x 10 -11 M Learn more about KD -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6513 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab248473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. -
Tissue specificity
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). -
Sequence similarities
Belongs to the eIF-6 family. -
Post-translational
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 3692 Human
- Entrez Gene: 16418 Mouse
- Entrez Gene: 305506 Rat
- Omim: 602912 Human
- SwissProt: P56537 Human
- SwissProt: O55135 Mouse
- SwissProt: Q3KRD8 Rat
- Unigene: 654848 Human
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Alternative names
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
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Datasheets and documents
References
ab248473 has not yet been referenced specifically in any publications.
