RecombinantRabMAb

Recombinant Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)

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Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
  • OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
  • Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6513] to eIF-6 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF-6 antibody [EPR6513] - BSA and Azide free
    See all eIF-6 primary antibodies

  • Description

    Rabbit monoclonal [EPR6513] to eIF-6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human eIF-6 aa 200-300 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab248473 is the carrier-free version of ab133321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248473 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function

      Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.

    • Tissue specificity

      Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

    • Sequence similarities

      Belongs to the eIF-6 family.

    • Post-translational
      modifications

      Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.

    • Cellular localization

      Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • b(2)gcn antibody
      • B(2)GCN homolog antibody
      • B4 integrin interactor antibody
      • Binding protein of beta-4 integrin antibody
      • CAB antibody
      • eIF-6 antibody
      • EIF3A antibody
      • EIF6 antibody
      • Eukaryotic translation initiation factor 3A antibody
      • Eukaryotic translation initiation factor 6 antibody
      • IF6_HUMAN antibody
      • Integrin beta 4 binding protein antibody
      • ITGB4BP antibody
      • OK/SW-cl.27 antibody
      • p27 beta 4 integrin binding protein antibody
      • p27(BBP) antibody
      • p27BBP antibody
      • RP4-614O4.1 antibody
      see all

    Images

    • Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
      Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
      All lanes : Anti-eIF-6 antibody [EPR6513] (ab133321) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 27 kDa



      This data was developed using ab133321, the same antibody clone in a different buffer formulation.

    • OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
      OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
      This data was developed using ab133321, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
      Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248473? Please let us know so that we can cite the reference in this datasheet.

    ab248473 has not yet been referenced specifically in any publications.

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