Recombinant
RabMAb

Recombinant Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

Overview

  • Product name

    Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free
    See all eIF1A primary antibodies
  • Description

    Rabbit monoclonal [EPR12466(B)] to eIF1A - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P47813

  • Positive control

    • IHC-P: Human endometrial carcinoma tissue; Human kidney tissue; ICC/IF: HeLa and 293T cells. FC: HeLa cells IP: HeLa cells
  • General notes

    Ab243919 is the carrier-free version of ab177939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243919 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12466(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    eIF1A is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA (referenced from entrez gene).
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • eIF-1A antibody
    • eIF-1A X isoform antibody
    • eIF-4C antibody
    • EIF1A antibody
    • EIF1AP1 antibody
    • EIF1AX antibody
    • EIF4C antibody
    • eukaryotic translation initiation factor 1A X-linked antibody
    • eukaryotic translation initiation factor 1A, X chromosome antibody
    • Eukaryotic translation initiation factor 1A, X-chromosomal antibody
    • eukaryotic translation initiation factor 4C antibody
    • Putative eukaryotic translation initiation factor 1A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium cancer tissue sections labeling eIF1A with Purified ab177939 at 1:350 dilution (1.61 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:100 dilution (5.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • ab177939 (purified) at 1:30 dilution (2µg) immunoprecipitating eIF1A in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab177939 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab177939 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Western blot analysis on immunoprecipitation pellet from A375 cell lysate immunoprecipitated using ab177939 at 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

  • Immunofluorescent analysis of HeLa cells labeling EIF1AX with ab177939 at 1/50 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EIF1AX with ab177939 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling EIF1AX with ab177939 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab243919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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