Product nameAnti-eIF1A antibody [EPR12467]
See all eIF1A primary antibodies
DescriptionRabbit monoclonal [EPR12467] to eIF1A
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chimpanzee, Orangutan
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P47813
- HeLa, 293T, Jurkat, and A375 cell lysates; Colonic carcinoma tissue; Pancreas tissue; A375 cells; Immunoprecipitation pellet from HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 16 kDa.|
RelevanceeIF1A is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA (referenced from entrez gene).
Cellular localizationCytoplasmic and Nuclear
- eIF-1A antibody
- eIF-1A X isoform antibody
- eIF-4C antibody
All lanes : Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.
Immunohistochemical analysis of paraffin-embedded pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.
Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)
This product has been referenced in:
- González-Almela E et al. The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells. Front Microbiol 9:207 (2018). Read more (PubMed: 29487587) »
- Yi T et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB, IP . Read more (PubMed: 26018492) »