Recombinant
RabMAb

Recombinant Anti-eIF1A antibody [EPR12467] (ab172623)

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Overview

  • Product name

    Anti-eIF1A antibody [EPR12467]
    See all eIF1A primary antibodies

  • Description

    Rabbit monoclonal [EPR12467] to eIF1A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chimpanzee, Orangutan

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P47813

  • Positive control

    • HeLa, 293T, Jurkat, and A375 cell lysates; Colonic carcinoma tissue; Pancreas tissue; A375 cells; Immunoprecipitation pellet from HeLa whole cell lysate (ab150035)

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12467

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
      Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
      All lanes : Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 16 kDa

    • Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

      Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)

      Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)

    References

    This product has been referenced in:

    • Del Puerto-Nevado L  et al. Diabetes-mediated promotion of colon mucosa carcinogenesis is associated with mitochondrial dysfunction. Mol Oncol 13:1887-1897 (2019). Read more (PubMed: 31199051) »
    • González-Almela E  et al. The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells. Front Microbiol 9:207 (2018). Read more (PubMed: 29487587) »
    See all 3 Publications for this product

    Publishing research using ab172623? Please let us know so that we can cite the reference in this datasheet.

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