RecombinantRabMAb

Recombinant Anti-eIF1A antibody [EPR12467] (ab172623)

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Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Anti-eIF1A antibody [EPR12467] (ab172623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12467] to eIF1A
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF1A antibody [EPR12467]
    See all eIF1A primary antibodies

  • Description

    Rabbit monoclonal [EPR12467] to eIF1A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chimpanzee, Orangutan

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P47813

  • Positive control

    • HeLa, 293T, Jurkat, and A375 cell lysates; Colonic carcinoma tissue; Pancreas tissue; A375 cells; Immunoprecipitation pellet from HeLa whole cell lysate (ab150035)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12467

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
      Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
      All lanes : Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 16 kDa

    • Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

      Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)
      Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)

      Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)

    • Anti-eIF1A antibody [EPR12467] (ab172623)
      Anti-eIF1A antibody [EPR12467] (ab172623)

    References (3)

    Publishing research using ab172623? Please let us know so that we can cite the reference in this datasheet.

    ab172623 has been referenced in 3 publications.

    • Del Puerto-Nevado L  et al. Diabetes-mediated promotion of colon mucosa carcinogenesis is associated with mitochondrial dysfunction. Mol Oncol 13:1887-1897 (2019). PubMed: 31199051
    • González-Almela E  et al. The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells. Front Microbiol 9:207 (2018). PubMed: 29487587
    • Yi T  et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB, IP . PubMed: 26018492

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