Anti-eIF1A antibody [EPR12467] (ab172623)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-eIF1A antibody [EPR12467]
See all eIF1A primary antibodies -
DescriptionRabbit monoclonal [EPR12467] to eIF1A
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chimpanzee, Orangutan
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P47813 -
Positive control
- HeLa, 293T, Jurkat, and A375 cell lysates; Colonic carcinoma tissue; Pancreas tissue; A375 cells; Immunoprecipitation pellet from HeLa whole cell lysate (ab150035)
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12467
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab172623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
Target
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RelevanceeIF1A is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA (referenced from entrez gene).
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 100986444 Chimpanzee
- Entrez Gene: 1964 Human
- Entrez Gene: 100172735 Orangutan
- Entrez Gene: 302697 Rat
- Omim: 300186 Human
- SwissProt: Q6GVM3 Chimpanzee
- SwissProt: P47813 Human
- SwissProt: Q60872 Mouse
see all -
Alternative names
- eIF-1A antibody
- eIF-1A X isoform antibody
- eIF-4C antibody
see all
Images
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![Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)](https://www.abcam.com/ps/products/172/ab172623/Images/ab172623-195802-5.jpg)
Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)All lanes : Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa -
![Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)](https://www.abcam.com/ps/products/172/ab172623/Images/ab172623-195801-4.jpg)
Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)](https://www.abcam.com/ps/products/172/ab172623/Images/ab172623-195800-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)Immunohistochemical analysis of paraffin-embedded pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)](https://www.abcam.com/ps/products/172/ab172623/Images/ab172623-195799-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)Immunohistochemical analysis of paraffin-embedded colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)](https://www.abcam.com/ps/products/172/ab172623/Images/ab172623-195798-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)
Protocols
Datasheets and documents
References
This product has been referenced in:
- González-Almela E et al. The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells. Front Microbiol 9:207 (2018). Read more (PubMed: 29487587) »
- Yi T et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB, IP . Read more (PubMed: 26018492) »
