Anti-EIF1AY antibody (ab155546)
Key features and details
- Rabbit polyclonal to EIF1AY
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EIF1AY antibody
See all EIF1AY primary antibodies -
Description
Rabbit polyclonal to EIF1AY -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-144 of Human EIF1AY (Uniprot ID: Q14602).
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Positive control
- Human Lung AdCA tissue; Raji and PC-12 whole cell lysate; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the eIF-1A family.
Contains 1 S1-like domain. - Information by UniProt
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Database links
- Entrez Gene: 9086 Human
- Entrez Gene: 302697 Rat
- Omim: 400014 Human
- SwissProt: O14602 Human
- Unigene: 461178 Human
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Alternative names
- eIF 1A Y isoform antibody
- eIF 4C antibody
- eIF-1A Y isoform antibody
see all
Images
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Anti-EIF1AY antibody (ab155546) at 1/500 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human Lung AdCA, labeling EIF1AY using ab155546 at 1/500 dilution.
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Anti-EIF1AY antibody (ab155546) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15 % SDS-PAGE -
Immunofluorescent analysis of methanol fixed A431 cells, labeling EIF1AY with ab155546 at 1/500 dilution (green). A431 cells were fixed in ice-cold Methanol for 5 min. Right panel shows Hoechst 33343 staining (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155546 has not yet been referenced specifically in any publications.